PXD040508-1
PXD040508 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Discovery of Itaconate-Mediated Lysine Acylation |
Description | Itaconate is an important anti-microbial and immunoregulatory metabolite involved in host-pathogen interactions. A key mechanistic action of itaconate is through the covalent modification of cysteine residues via Michael addition, resulting in “itaconation”. However, it is unclear whether itaconate has other regulatory mechanisms. In this work, we discovered a novel type of post-translational modification by promiscuous antibody enrichment and data analysis with the open-search strategy, and further confirmed it as the lysine “itaconylation”. We showed that itaconylation undergo significant up-regulation upon lipopolysaccharides (LPS) stimulation in RAW264.7 macrophages. Quantitative proteomics identified itaconylation sites in several functional proteins, including glycolytic enzymes and histones, some of which were confirmed by synthetic peptide standards. |
HostingRepository | iProX |
AnnounceDate | 2023-02-28 |
AnnouncementXML | Submission_2023-06-07_00:30:10.784.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dongyang Liu |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-02-28 18:32:51 | ID requested | |
⏵ 1 | 2023-06-07 00:30:11 | announced |
Publication List
Liu D, Xiao W, Li H, Zhang Y, Yuan S, Li C, Dong S, Wang C, Discovery of Itaconate-Mediated Lysine Acylation. J Am Chem Soc, 145(23):12673-12681(2023) [pubmed] |
Keyword List
submitter keyword: itaconylation, itaconate, post-translational modification |
Contact List
Chu Wang | |
---|---|
contact affiliation | Peking University |
contact email | chuwang@pku.edu.cn |
lab head | |
Dongyang Liu | |
contact affiliation | Peking University |
contact email | sobeit@pku.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |