PXD040497 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Vacuum insulated probe heated electrospray ionization source (VIP-HESI) significantly enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer. |
Description | Nano-flow chromatography-tandem mass spectrometer (nLC-MS/MS) is a popular choice in proteome research as it provided high-sensitivity analysis with minimal sample usage, but its quantitative proteomics applications become restricted by low analytical throughput, and low robustness. To circumvent this limitation, we describe the performance of the new Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) coupled to the Bruker timsTOF mass spectrometer and show it enhances micro-spray flow rate chromatography signals in comparison to nanospray source conditions using the CaptiveSpray (CS) and ElectroSpray Ionization (ESI) sources. In addition, using a 0.5 x 200mm and 1 × 150 mm columns, achieved excellent reproducibility of chromatographic retention time, coefficient of variation, and precision quantification using data from un-depleted mouse plasma, HeLa and K562 digest peptide samples. |
HostingRepository | PRIDE |
AnnounceDate | 2023-05-25 |
AnnouncementXML | Submission_2023-05-25_10:12:36.593.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MukulMidha |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-28 05:17:55 | ID requested | |
⏵ 1 | 2023-05-25 10:12:37 | announced | |
Publication List
Midha MK, Kapil C, Maes M, Baxter DH, Morrone SR, Prokop TJ, Moritz RL, Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer. bioRxiv, ():(2023) [pubmed] |
Keyword List
submitter keyword: slice-PASEF, Electrospray ionization (ESI), dia-PASEF, Micro-flow rate chromatography,Vacuum insulated probe heated electrospray ionization source (VIP-HESI), Captive Spray (CS) |
Contact List
Robert L.Moritz |
contact affiliation | Lab head, Director Proteomics Group, Institute for Systems Biology (ISB), Seattle, WA, USA |
contact email | rmoritz@systemsbiology.org |
lab head | |
MukulMidha |
contact affiliation | Institute for Systems Biology |
contact email | mmidha@systemsbiology.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040497
- Label: PRIDE project
- Name: Vacuum insulated probe heated electrospray ionization source (VIP-HESI) significantly enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.