PXD040497-1

PXD040497 is an original dataset announced via ProteomeXchange.

Title	Vacuum insulated probe heated electrospray ionization source (VIP-HESI) significantly enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.
Description	Nano-flow chromatography-tandem mass spectrometer (nLC-MS/MS) is a popular choice in proteome research as it provided high-sensitivity analysis with minimal sample usage, but its quantitative proteomics applications become restricted by low analytical throughput, and low robustness. To circumvent this limitation, we describe the performance of the new Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) coupled to the Bruker timsTOF mass spectrometer and show it enhances micro-spray flow rate chromatography signals in comparison to nanospray source conditions using the CaptiveSpray (CS) and ElectroSpray Ionization (ESI) sources. In addition, using a 0.5 x 200mm and 1 × 150 mm columns, achieved excellent reproducibility of chromatographic retention time, coefficient of variation, and precision quantification using data from un-depleted mouse plasma, HeLa and K562 digest peptide samples.
HostingRepository	PRIDE
AnnounceDate	2023-05-25
AnnouncementXML	Submission_2023-05-25_10:12:36.593.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MukulMidha
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-28 05:17:55	ID requested
⏵ 1	2023-05-25 10:12:37	announced

Midha MK, Kapil C, Maes M, Baxter DH, Morrone SR, Prokop TJ, Moritz RL, Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer. bioRxiv, ():(2023) [pubmed]

submitter keyword: slice-PASEF, Electrospray ionization (ESI), dia-PASEF, Micro-flow rate chromatography,Vacuum insulated probe heated electrospray ionization source (VIP-HESI), Captive Spray (CS)

Robert L.Moritz
contact affiliation	Lab head, Director Proteomics Group, Institute for Systems Biology (ISB), Seattle, WA, USA
contact email	rmoritz@systemsbiology.org
lab head
MukulMidha
contact affiliation	Institute for Systems Biology
contact email	mmidha@systemsbiology.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040497
     1. Label: PRIDE project
     2. Name: Vacuum insulated probe heated electrospray ionization source (VIP-HESI) significantly enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.