<<< Full experiment listing


PXD040497 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVacuum insulated probe heated electrospray ionization source (VIP-HESI) significantly enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer.
DescriptionNano-flow chromatography-tandem mass spectrometer (nLC-MS/MS) is a popular choice in proteome research as it provided high-sensitivity analysis with minimal sample usage, but its quantitative proteomics applications become restricted by low analytical throughput, and low robustness. To circumvent this limitation, we describe the performance of the new Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) coupled to the Bruker timsTOF mass spectrometer and show it enhances micro-spray flow rate chromatography signals in comparison to nanospray source conditions using the CaptiveSpray (CS) and ElectroSpray Ionization (ESI) sources. In addition, using a 0.5 x 200mm and 1 × 150 mm columns, achieved excellent reproducibility of chromatographic retention time, coefficient of variation, and precision quantification using data from un-depleted mouse plasma, HeLa and K562 digest peptide samples.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-28 05:17:55ID requested
12023-05-25 10:12:37announced
22023-11-14 08:48:18announced2023-11-14: Updated project metadata.
Publication List
Midha MK, Kapil C, Maes M, Baxter DH, Morrone SR, Prokop TJ, Moritz RL, Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer. bioRxiv, ():(2023) [pubmed]
Keyword List
submitter keyword: slice-PASEF, Electrospray ionization (ESI), dia-PASEF, Micro-flow rate chromatography,Vacuum insulated probe heated electrospray ionization source (VIP-HESI), Captive Spray (CS)
Contact List
Robert L.Moritz
contact affiliationLab head, Director Proteomics Group, Institute for Systems Biology (ISB), Seattle, WA, USA
contact emailrmoritz@systemsbiology.org
lab head
contact affiliationInstitute for Systems Biology
contact emailmmidha@systemsbiology.org
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD040497
PRIDE project URI
Repository Record List
[ + ]