PXD040467-1

PXD040467 is an original dataset announced via ProteomeXchange.

Title	Metabolic influence of core ciliates within the rumen microbiome
Description	Protozoa comprise a major fraction of the microbial biomass in the rumen microbiome, of which the entodiniomorphs (order: Entodiniomorphida) and holotrichs (order: Vestibuliferida) are consistently observed to be dominant across a diverse genetic and geographical range of ruminant hosts. Despite the apparent core role that protozoal species exert, their major biological and metabolic contributions to rumen function remain largely undescribed in vivo. Here, we have leveraged (meta)genome27 centric metaproteomes from rumen fluid samples originating from both cattle and goats fed diets with varying inclusion levels of lipids and starch, to detail the specific metabolic niches that protozoa occupy in the context of their microbial co-habitants. Initial proteome estimations via total protein counts and label-free quantification highlight that entodiniomorph species Entodinium and Epidinium as well as the holotrichs Dasytricha and Isotricha comprises an extensive fraction of the total rumen metaproteome. Proteomic detection of protozoal metabolism such as hydrogenases (Dasytricha, Isotricha, Epidinium, Enoploplastron), carbohydrate-active enzymes (Epidinium, Diplodinium, Enoploplastron, Polyplastron), microbial predation (Entodinium) and volatile fatty acid production (Entodinium and Epidinium) was observed at increased levels in high methane-emitting animals. Despite certain protozoal species having well-established reputations for digesting starch, they were unexpectedly less detectable in low methane emitting- 37 animals fed high starch diets, which were instead dominated by propionate/succinate-producing bacterial populations suspected of being resistant to predation irrespective of host. Finally, we reaffirmed our abovementioned observations in geographically independent datasets, thus illuminating the substantial metabolic influence that under-explored eukaryotic populations have in the rumen, with greater implications for both digestion and methane metabolism.
HostingRepository	PRIDE
AnnounceDate	2023-04-19
AnnouncementXML	Submission_2023-04-19_01:47:40.971.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TheaAndersen
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 07:40:50	ID requested
⏵ 1	2023-04-19 01:47:41	announced
2	2023-11-14 08:59:35	announced	2023-11-14: Updated project metadata.

submitter keyword: metaproteomics, microbiome, metagenomics, rumen, protozoa

Dr. Phiilip B.Pope
contact affiliation	Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences Post Office Box 5003 1432, Ås, Norway
contact email	phil.pope@nmbu.no
lab head
TheaAndersen
contact affiliation	Norwegian University of Life Sciences
contact email	thea.os.andersen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040467
     1. Label: PRIDE project
     2. Name: Metabolic influence of core ciliates within the rumen microbiome