PXD040458-2

PXD040458 is an original dataset announced via ProteomeXchange.

Title	Photophthora sojae extracellular vesicles proteins
Description	Extracellular vesicles (EVs) have been extensively studied in animal cells, and play an important role in cell-to-cell communications. Emerging evidence shows that EVs also act as important agents in plant-microbe interactions. However, the mechanisms by which EVs mediate cross-kingdom interactions between plants and microbial pathogens remain largely elusive. Here, proteomic analyses of soybean root rot pathogen Phytophthora sojae EVs identified tetraspanin family proteins, PsTET1 and PsTET3, that can be recognized by Nicotiana benthamiana to trigger plant immune responses. PsTET1 and PsTET3 were redundantly required for the full virulence of P. sojae. Further analyses revealed that the large extracellular loop (EC2) of PsTET3 is the key region recognized by N. benthamiana and also by Glycine max, and that recognition depends on the plant receptor-like kinase BAK1. TET proteins from oomycete and fungal plant pathogens could be recognized by N. benthamiana and induce immune responses. However, plant-derived TET proteins failed to do so, due to the divergent sequences of the final 16 amino acids of EC2, which ultimately makes plants distinguish self and non-self EVs, triggering active defenses against pathogenic eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:24.435.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinyi Zhu
SpeciesList	scientific name: Phytophthora sojae strain P6497; NCBI TaxID: 1094619;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 06:16:06	ID requested
1	2023-07-31 23:49:40	announced
⏵ 2	2024-10-22 05:59:24	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: photophthora sojae, LC-MS, extracellular vesicles

Yuanchao Wang
contact affiliation	Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
contact email	wangyc@njau.edu.cn
lab head
Jinyi Zhu
contact affiliation	Nanjing Agriculture University
contact email	2018202021@njau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD040458

PRIDE project URI

• PRIDE
  1. PXD040458
     1. Label: PRIDE project
     2. Name: Photophthora sojae extracellular vesicles proteins