PXD040456-1

PXD040456 is an original dataset announced via ProteomeXchange.

Title	mRNA 3’UTR lengthening by alternative polyadenylation attenuates inflammatory responses and correlates with virulence of Influenza A virus
Description	Alternative polyadenylation (APA) leading to changes of mRNA 3’UTRs has been associated to numerous pathologies but its molecular origin and functional consequences often remain enigmatic. We show that all Influenza A virus (IAV) strains cause APA of host transcripts and mapped the effect to a drug-targetable glycine residue (G184) in the viral non-structural protein 1 (NS1) that is required for its interaction with CPSF4. Unexpectedly, this interaction is also conserved in IAVs not causing virus-induced transcriptional shutoff and introduction of G184R in NS1 alleviates APA. Functionally, a recombinant PR8 (NS1-G184R) virus elicited increased secretion of inflammatory cytokines as compared to the parental wild-type in vitro and in vivo, and had a strongly attenuated phenotype in vivo. Finally, we show that many pathogens induce APA, which is potentially used towards similar immunomodulatory effects.
HostingRepository	PRIDE
AnnounceDate	2023-07-12
AnnouncementXML	Submission_2023-07-12_06:33:05.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ValterBergant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 06:15:30	ID requested
⏵ 1	2023-07-12 06:33:06	announced

Dataset with its publication pending

submitter keyword: IAV, NS1,Semliki Forest Virus, PR8, SFV, Influenza A

AndreasPichlmair
contact affiliation	Immunopathology of Virus Infections Institute of Virology Technical University of Munich
contact email	Andreas.pichlmair@tum.de
lab head
ValterBergant
contact affiliation	Institute of Virology, School of Medicine, Technical University of Munich (TUM), Munich, Germany
contact email	v.bergant@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD040456

PRIDE project URI

• PRIDE
  1. PXD040456
     1. Label: PRIDE project
     2. Name: mRNA 3’UTR lengthening by alternative polyadenylation attenuates inflammatory responses and correlates with virulence of Influenza A virus