PXD040452-2

PXD040452 is an original dataset announced via ProteomeXchange.

Title	Proximity interactome of lymphatic VE-cadherin reveals mechanisms of junctional remodeling and reelin secretion.
Description	In this study, we are the first to use the MiniTurboID generation of the proximity-dependent biotin identification (BioID) technology to illuminate how the lymphatic endothelial cell (LEC) VE-cadherin interactome changes during junctional remodeling in response to the lymphangiocrine factor adrenomedullin. Here, we created a UBC-driven lentiviral expression vector containing the coding sequence for a Ve-Cadehrin-V5-MiniTurboID fusion-protein. After transduction of LECs with Ve-Cadehrin-MiniTurboID lentivirus, we treated these cells with 100 nM adrenomedullin (AM) or vehicle and labeled proximal proteins with 50 µM for 2 hours to capture dynamic changes in the Ve-Cadehrin interactome during AM-induced junctional rearrangement. Biotin-labeled proximity interactors were isolated via streptavidin-affinity purification (AP) and identified using LC-MS/MS mass spectrometry. Our dataset consists of distinct biological replicates consisting of AP enriched proteins from whole cell lysates (WCL, n = 2) or plasma membrane (PM, n = 3) fractions. WCL fractions allow us to capture proximity networks involved in Ve-Cadehrin trafficking and recycling to the PM, while PM fractions allows us to examine membrane-specific changes in AM-induced adherens junctions assembly.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:42.929.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine Mills
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	mono N-acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 06:13:42	ID requested
1	2024-09-05 06:50:03	announced
⏵ 2	2024-10-22 06:57:43	announced	2024-10-22: Updated project metadata.

10.1038/S41467-024-51918-1;

submitter keyword: VE-cadherin, Exocyst, Lymphatic Endothelial Cells, Adherens Junctions, Reelin, BioID

Kathleen Caron
contact affiliation	Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill
contact email	kathleen_caron@med.unc.edu
lab head
Christine Mills
contact affiliation	University of North Carolina - Chapel Hill
contact email	allie.mills@unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD040452

PRIDE project URI

• PRIDE
  1. PXD040452
     1. Label: PRIDE project
     2. Name: Proximity interactome of lymphatic VE-cadherin reveals mechanisms of junctional remodeling and reelin secretion.