PXD040423 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Label-free quantitative proteomics analysis of bone marrow cells of MK-LCM WT versus KO mice |
Description | High ploidy large cytoplasmic megakaryocytes (LCM) are critical negative regulators of hematopoietic stem cells (HSC) and are responsible for platelet formation. Using a mouse knockout model with normal megakaryocyte numbers but essentially devoid of LCM (MK-LCM KO), we demonstrated a pronounced increase in bone marrow HSC concurrent with endogenous mobilization and extramedullary hematopoiesis. When HSC isolated from a MK-LCM KO microenvironment were transplanted in lethally irradiated mice, the absence of LCM increased HSC in BM, blood and spleen. Severe thrombocytopenia was observed in animals with diminished LCM, although there was no change in megakaryocyte ploidy distribution. In contrast, WT HSC-generated LCM regulated a normal HSC pool and prevented thrombocytopenia. The present label-free quantitative LC-MSMS data was used to determine proteins that are differentially expressed in bone marrow cells of MK-LCM WT versus MK-LCM KO mice. |
HostingRepository | PRIDE |
AnnounceDate | 2023-05-10 |
AnnouncementXML | Submission_2023-05-10_14:47:15.206.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ThomasNebl |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-27 05:18:20 | ID requested | |
⏵ 1 | 2023-05-10 14:47:16 | announced | |
2 | 2023-11-14 08:47:53 | announced | 2023-11-14: Updated project metadata. |
Publication List
Heazlewood SY, Ahmad T, Cao B, Cao H, Domingues M, Sun X, Heazlewood CK, Li S, Williams B, Fulton M, White JF, Nebl T, Nefzger CM, Polo JM, Kile BT, Kraus F, Ryan MT, Sun YB, Choong PFM, Ellis SL, Anko ML, Nilsson SK, High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production. Nat Commun, 14(1):2099(2023) [pubmed] |
Keyword List
submitter keyword: LC-MSMS, bone marrow,Mouse, LFQ |
Contact List
TomNebl |
contact affiliation | Biologics R&D Group/ Biomedical Program CSIRO Manufacturing Bayview Ave/ Research Way, Clayton, VIC3168 Australia |
contact email | tom.nebl@csiro.au |
lab head | |
ThomasNebl |
contact affiliation | CSIRO |
contact email | tom.nebl@csiro.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040423
- Label: PRIDE project
- Name: Label-free quantitative proteomics analysis of bone marrow cells of MK-LCM WT versus KO mice