PXD040419-1

PXD040419 is an original dataset announced via ProteomeXchange.

Title	Ancient palaeoimmunology: diving deeper with a two enzyme proteomics approach
Description	This study investigates the efficacy of proteomic analysis of human remains to identify active Mycobacterium leprae infections in the past. Mycobacterial diseases, like leprosy, have plagued human populations for millennia. Thanks to effective treatment options, leprosy is not as widespread and deadly as in the past, yet remains endemic in certain regions with increasing concerns of strains becoming resistant to antibiotic treatments. We present a dual-enzyme, optimised extraction protocol, using trypsin and ProAlanase, to increase the recovery of non-collagenous proteins through a study of five individuals from a Mediaeval leprosarium cemetery, as well as four from a non-leprosy associated cemetery. Here we show that skeletal samples from the leprosarium individuals contain numerous immune proteins associated with modern leprosy, while those from a non-leprosy associated cemetery do not. Through this study, we advance a palaeoimmunology methodology and provide insights into the health of archaeological individuals and offer a means to triage samples for aDNA analysis.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_02:17:00.948.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shevan Wilkin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 05:12:50	ID requested
⏵ 1	2024-05-24 02:17:01	announced

10.1016/j.isci.2024.109663;

Wilkin S, Lanigan LT, Montes N, Sharma M, Avanzi C, Sejdiu D, Majander K, Pfrengle S, Chiang Y, Kunz L, Dittmann A, R, ü, hli F, Singh P, Coll MF, Collins MJ, Taurozzi AJ, Schuenemann VJ, Sequential trypsin and ProAlanase digestions unearth immunological protein biomarkers shrouded by skeletal collagen. iScience, 27(5):109663(2024) [pubmed]

submitter keyword: Ancient proteins, leprosy, immune response, paleoimmunoproteomics, LC-MS/MS

Dr. Shevan Wilkin
contact affiliation	Institute of Evolutionary Medicine, University of Zurich
contact email	shevanwilkin@me.com
lab head
Shevan Wilkin
contact affiliation	Max Planck Institute for the Science of Human History
contact email	wilkin@shh.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD040419

PRIDE project URI

• PRIDE
  1. PXD040419
     1. Label: PRIDE project
     2. Name: Ancient palaeoimmunology: diving deeper with a two enzyme proteomics approach