PXD040414-3

PXD040414 is an original dataset announced via ProteomeXchange.

Title	Nicotine administration augments abdominal aortic aneurysm progression in rats
Description	Inflammation and elastin degradation are key hallmarks in the pathogenesis of abdominal aortic aneurysms (AAA). It has been acknowledged that activation of alpha7 nicotinic acetylcholine receptors (α7nAChRs) attenuates inflammation, termed the cholinergic anti-inflammatory pathway (CAP). Thus, we hypothesized that low-dose nicotine, an α7nAChR agonist, impairs the progression of AAAs in rats by activating the CAP. Male Sprague-Dawley rats underwent surgical AAA induction with intraluminal elastase infusion. We compared vehicle rats with rats treated with nicotine (1.25 mg/kg/day) and the aneurysm progression was monitored by weekly ultrasound images for 28 days. Nicotine significantly promoted AAA progression after 28 days of treatment (p=0.031). Additionally, gelatin zymography demonstrated that nicotine significantly reduced pro-matrix metalloprotease (pro-MMP) 2(p=0.029) and MMP9(p=0.030) activity in aneurysmal tissue. No significant difference was found in elastin content or the score of elastin degradation between the groups. Neither infiltrating neutrophils nor macrophages, nor aneurysmal messenger RNA (mRNA) levels of pro- or anti-inflammatory cytokines differed between the vehicle and nicotine group. Finally, no difference in mRNA levels of markers for antioxidative stress or vascular smooth muscle cells contractile phenotype was observed. However, proteomics analyses of non-aneurysmal abdominal aortas revealed that nicotine decreased proteins in the ontology terms inflammation and reactive oxygen species and in contradiction to augmented AAAs. In conclusion, treatment with the given nicotine dose augmented AAA progression in this rat model.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:56:28.862.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hans Christian Beck
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 03:12:02	ID requested
1	2023-07-20 11:38:14	announced
2	2023-11-14 09:03:26	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:56:31	announced	2024-10-22: Updated project metadata.

Hadzikadunic H, Sj, æ, lland TB, Lindholt JS, Steffensen LB, Beck HC, Kavaliunaite E, Rasmussen LM, Stubbe J, Nicotine Administration Augments Abdominal Aortic Aneurysm Progression in Rats. Biomedicines, 11(5):(2023) [pubmed]

10.3390/biomedicines11051417;

submitter keyword: therapeutic strategy, inflammation, aortic aneurysm,Proteomics, alpha7 nicotinic acetylcholine receptor, vascular remodeling, nicotine, animal model

Hans Christian Beck
contact affiliation	Proteomics Lab, Dept. Clinical Biochemistry, Odense University Hospital
contact email	hans.christian.beck@rsyd.dk
lab head
Hans Christian Beck
contact affiliation	Odense University Hospital, Odense, Denmark
contact email	hans.christian.beck@rsyd.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040414
     1. Label: PRIDE project
     2. Name: Nicotine administration augments abdominal aortic aneurysm progression in rats