PXD040383-2

PXD040383 is an original dataset announced via ProteomeXchange.

Title	The role of serine/threonine-specific protein kinases in cyanobacteria - SpkB is involved in acclimation to fluctuating conditions in Synechocystis sp. PCC 6803
Description	Protein phosphorylation via serine/threonine protein kinases (Spk) is a widespread mechanism to adjust cellular processes toward changing environmental conditions. To study their role(s) in cyanobacteria, we established a collection of 11 completely segregated spk mutants among the 12 annotated Spk’s in the model cyanobacterium Synechocystis sp. PCC 6803. Screening of the mutant collection revealed that especially the mutant defective in SpkB encoded by slr1697 showed clear deviations compared to wild type (WT) regarding carbon metabolism, i.e., a reduced growth rate at low CO2 and in the presence of glucose, different glycogen accumulation patterns, and a higher tolerance to external H2O2 than the WT. The proteome of ∆spkB showed several distinct differences compared to WT, which indicate changes of the cell surface but also metabolic functions. A phospho-proteome analysis revealed decreased phosphorylation of the carboxysome-associated protein CcmM and the regulatory PII protein in the mutant compared to WT, whereas the allophycocyanin alpha subunit was stronger phosphorylated. The decreased phosphorylation of PII was verified in Western-blot experiments, indicating a clearly delayed PII phosphorylation in cells shifted from nitrate-containing to nitrate-free medium. Furthermore, the mutant ∆spkB showed differences in the state transition consistent with the changed phosphorylation of allophycocyanin. Collectively our results indicate that SpkB is an important regulator under different environmental conditions in Synechocystis and seems to interact in the PII phosphorylation and probably with further substrates in a kinase network.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:27:05.624.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philipp Spaet
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-24 04:29:07	ID requested
1	2024-01-26 09:32:00	announced
⏵ 2	2024-10-22 06:27:06	announced	2024-10-22: Updated project metadata.

Barske T, Sp, ä, t P, Schubert H, Walke P, Ma, č, ek B, Hagemann M, The Role of Serine/Threonine-Specific Protein Kinases in Cyanobacteria - SpkB Is Involved in Acclimation to Fluctuating Conditions in Synechocystis sp. PCC 6803. Mol Cell Proteomics, 22(11):100656(2023) [pubmed]

10.1016/j.mcpro.2023.100656;

submitter keyword: adaptation,Cyanobacteria, kinases, phosphorylation, Synechocystis sp. PCC6803, SpkB

Prof. Dr. Martin Hagemann
contact affiliation	Institut für Biowissenschaften, Abteilung Pflanzenphysiologie, Universität Rostock, A.-Einstein-Str. 3, Rostock D-18059, Germany
contact email	martin.hagemann@uni-rostock.de
lab head
Philipp Spaet
contact affiliation	University Tuebingen Department of Quantitative Proteomics
contact email	philipp.spaet@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040383
     1. Label: PRIDE project
     2. Name: The role of serine/threonine-specific protein kinases in cyanobacteria - SpkB is involved in acclimation to fluctuating conditions in Synechocystis sp. PCC 6803