PXD040356-1

PXD040356 is an original dataset announced via ProteomeXchange.

Title	Identification of pspA-3’UTR-regulated proteins by quantitative proteomics
Description	RNA-mediated control of virulence gene expression is widespread among pathogenic bacteria. However, in some major human pathogens such as Streptococcus pneumoniae, its role remains poorly understood. In this study we identify a regulatory function for the 3’-untranslated region (3’UTR) of the mRNA encoding the pneumococcal surface protein A (PspA), a multifunctional surface protein and major virulence factor. Using quantitative proteomics, we identify the protein chaperone Caseinolytic protease L (ClpL) as a regulatory target of the pspA-3’UTR. Downstream analysis reveals that the pspA-3’UTR represses ClpL expression in a temperature-dependent manner. Altogether, this study unveils a riboregulatory role for the pspA mRNA, revealing another layer of PspA-mediated virulence in the human pathogen S. pneumoniae.
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_05:20:07.198.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Pettersen
SpeciesList	scientific name: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466); NCBI TaxID: NEWT:373153;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-23 03:52:13	ID requested
⏵ 1	2026-05-27 05:20:08	announced

Pettersen JS, Lund A, Kirpekar F, Hansen NV, Svenningsen SL, Paton JC, M, ø, ller-Jensen J, J, ø, rgensen MG, 3' UTR-mediated regulation of a protein chaperone by the pspA mRNA in Streptococcus pneumoniae. Nucleic Acids Res, 54(9):(2026) [pubmed]

10.1093/nar/gkag481;

submitter keyword: LC-MSMS, Streptococcus pneumoniae, TMT10plex,Pathogenic bacteria, Regulatory RNAs

Mikkel Girke Jørgensen
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark
contact email	mikkelj@bmb.sdu.dk
lab head
Jens Pettersen
contact affiliation	Deparment of Biochemistry and Molecular Biology, University of Southern Denmark
contact email	sivkaer@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD040356

PRIDE project URI

• PRIDE
  1. PXD040356
     1. Label: PRIDE project
     2. Name: Identification of pspA-3’UTR-regulated proteins by quantitative proteomics