PXD040338 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona |
Description | Correct chromosome segregation during cell division depends on proper connections between spindle microtubules and kinetochores. During prometaphase, kinetochores are temporarily covered with a dense protein meshwork known as the fibrous corona. Formed by oligomerization of ROD/ZW10/ZWILCH-SPINDLY (RZZ-S) complexes, the fibrous corona promotes spindle assembly, chromosome orientation and spindle checkpoint signaling. The molecular requirements for formation of the fibrous corona are not fully understood. Here we show that the fibrous corona depends on the mitotic kinesin CENP-E, and that poorly expanded fibrous coronas after CENP-E depletion are functionally compromised. This previously unrecognized role for CENP-E does not require its motor activity but instead is driven by its C-terminal microtubule-binding region and farnesyl modification thereof. The ‘birth’ of a farnesylation motif in CENP-E orthologs during Obazoa evolution closely aligns with presence of the full RZZ-S module, and we show that in cells CENP-E interacts with RZZ-S complexes in a farnesyl-dependent manner. CENP-E is recruited to kinetochores following RZZ-S, and - while not required for RZZ-S oligomerization per se - promotes subsequent fibrous corona expansion. Our results show that proper spindle assembly has a non-motor contribution from the kinesin CENP-E through stabilization of the fibrous corona meshwork during its formation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-09 |
AnnouncementXML | Submission_2023-10-09_03:03:25.692.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | HarmjanVos |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-22 10:19:53 | ID requested | |
⏵ 1 | 2023-10-09 03:03:26 | announced | |
2 | 2024-10-22 06:05:15 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: CENP-E, farnesylation, kinetochores,Chromosome segregation, ROD/ZW10/ZWILCH-SPINDLY (RZZ-S) complex |
Contact List
J.P.L.Kops |
contact affiliation | Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), Uppsalalaan 8, 3584CT, Utrecht, the Netherlands. |
contact email | g.kops@hubrecht.eu |
lab head | |
HarmjanVos |
contact affiliation | University Medical Center Utrecht Dept. Molecular Cancer Research |
contact email | h.r.vos-3@umcutrecht.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040338
- Label: PRIDE project
- Name: A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona