PXD040303 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structural basis of activation and inhibition of the Ca2+/calmodulin-sensitive adenylyl cyclase 8 (LiP-MS) |
Description | Membrane adenylyl cyclases (ACs) catalyze the conversion of ATP to cAMP, a second messenger involved in different signaling pathways. AC8 is one of the 9 membrane-bound isoforms, present in the brain and implicated in cognitive functions. AC8 is regulated by Ca2+/CaM and different G proteins but structural evidence on its regulation is scarce. We solved the structure of full-length AC8 in complex with Gas and CaM. ACs contain large stretches of disordered/highly flexible domains that cannot be resolved with cryo-EM. To overcome this limitation, we have studied AC8's interaction with Gas and Gbg using limited proteolysis-coupled mass spectrometry (LiP-MS). A previously published data set on the interaction of AC8 and CaM was included in the analysis (PXD039520). |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-14 |
AnnouncementXML | Submission_2024-02-14_08:19:34.315.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dina Schuster |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-21 02:42:56 | ID requested | |
⏵ 1 | 2024-02-14 08:19:34 | announced | |
Publication List
Keyword List
submitter keyword: structural proteomics,LiP-MS, membrane protein |
Contact List
Volodymyr M. Korkhov |
contact affiliation | Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institute, Villigen, Switzerland; Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zurich, Switzerland |
contact email | volodymyr.korkhov@psi.ch |
lab head | |
Dina Schuster |
contact affiliation | ETH Zurich |
contact email | dschuster@ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040303
- Label: PRIDE project
- Name: Structural basis of activation and inhibition of the Ca2+/calmodulin-sensitive adenylyl cyclase 8 (LiP-MS)