PXD040264-1

PXD040264 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	UBOX-UBL versus UBOX-UBLcontrol tranfected into HeLa Cells
Description	In this work, we developed a suite of linked-domain UBE2D inhibitors. We transfected these constructs into HeLa cells and used shotgun proteomics with label-free quantification to measure which protein's abundances are affected by UBE2D inhibition.
HostingRepository	PRIDE
AnnounceDate	2025-12-12
AnnouncementXML	Submission_2025-12-11_17:04:13.738.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joseph Harrison
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-20 04:54:21	ID requested
⏵ 1	2025-12-11 17:04:14	announced

Publication List

10.1016/j.chembiol.2025.11.007;
Bukhari Z, Gu L, Nederstigt AE, Cope LJ, Bolhuis DL, Harvey K, Allen T, Hill S, Yang Y, Lawson G, Lu C, Tran T, Pineda L, Low L, Chiang A, Song J, Fong MV, Rangel VM, Chan WK, Kleiger G, Goldfarb D, Vierra CA, Brown NG, Harrison JS, Linked-domain inhibitors designed to block UBE2D induce the unfolded protein response. Cell Chem Biol, 32(12):1532-1544.e7(2025) [pubmed]

10.1016/j.chembiol.2025.11.007;

Bukhari Z, Gu L, Nederstigt AE, Cope LJ, Bolhuis DL, Harvey K, Allen T, Hill S, Yang Y, Lawson G, Lu C, Tran T, Pineda L, Low L, Chiang A, Song J, Fong MV, Rangel VM, Chan WK, Kleiger G, Goldfarb D, Vierra CA, Brown NG, Harrison JS, Linked-domain inhibitors designed to block UBE2D induce the unfolded protein response. Cell Chem Biol, 32(12):1532-1544.e7(2025) [pubmed]

Keyword List

submitter keyword: ube2D, E2,ubiquitin

submitter keyword: ube2D, E2,ubiquitin

Contact List

Joseph S.
contact affiliation	University of the Pacific
contact email	jharrison@pacific.edu
lab head
Joseph Harrison
contact affiliation	University of the Pacific
contact email	jharrison@pacific.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD040264
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD040264

PRIDE project URI

Repository Record List

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