PXD040262-1

PXD040262 is an original dataset announced via ProteomeXchange.

Title	A Spatiotemporal Map of Co-Receptor Signaling Networks Underlying B Cell Activation
Description	The B cell receptor (BCR) signals together with a multi-component co-receptor complex to initiate B cell activation in response to antigen binding. This process underlies nearly every aspect of proper B cell function. Here, we take advantage of peroxidase-catalyzed proximity labeling combined with quantitative mass spectrometry to track B cell co-receptor signaling dynamics from 10 seconds to 2 hours after BCR stimulation. This approach enables tracking of 2,814 proximity-labeled proteins and 1,394 quantified phosphosites and provides an unbiased and quantitative molecular map of proteins recruited to the vicinity of CD19, the key signaling subunit of the co-receptor complex. We detail the recruitment kinetics of essential signaling effectors to CD19 following activation, and then identify new mediators of B cell activation. In particular, we show that the glutamate transporter SLC1A1 is responsible for mediating rapid metabolic reprogramming immediately downstream of BCR stimulation and for maintaining redox homeostasis during B cell activation. This study provides a comprehensive map of the BCR signaling pathway and a rich resource for uncovering the complex signaling networks that regulate B cell activation.
HostingRepository	PRIDE
AnnounceDate	2024-06-06
AnnouncementXML	Submission_2024-06-06_13:47:11.222.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gary Bradshaw
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-20 04:53:03	ID requested
⏵ 1	2024-06-06 13:47:11	announced

Dataset with its publication pending

submitter keyword: systems immunology, proteomics, BCR signaling, B cell co-receptor,B cells, phosphoproteomics

Andrew C. Kruse
contact affiliation	Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA
contact email	andrew_kruse@hms.harvard.edu
lab head
Gary Bradshaw
contact affiliation	Harvard Medical School
contact email	Gary_bradshaw@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD040262

PRIDE project URI

• PRIDE
  1. PXD040262
     1. Label: PRIDE project
     2. Name: A Spatiotemporal Map of Co-Receptor Signaling Networks Underlying B Cell Activation