PXD040225-1

PXD040225 is an original dataset announced via ProteomeXchange.

Title	Retinal Pathological Features and Proteome Signatures of Alzheimer’s Disease
Description	Alzheimer’s disease (AD) pathologies were discovered in the easily accessible neurosensory retina. Yet, their specific nature, topographical distribution, and relationship with disease status remain undefined. Here, we histologically determined burden and spatial distribution of amyloid β-protein (Aβ42 ), intraneuronal scFvA13 + -Aβ species, macro- and microgliosis, and atrophy in superior- and inferiortemporal retinas of human donors with mild cognitive impairment (MCI) or AD versus normal cognition. AD and MCI patients had enhanced retinopathy, predominantly affecting inner layers and peripheral subregions, which quantitatively correlated with severity of cerebral amyloid, tau, and neurodegeneration, and cognitive scores. In advanced clinical stages AD retinopathy further affected central outer segments. Increased retinal macrogliosis and Aβ-phagocytosing microglia were detected in MCI and AD patients. Further, distinct proteome profiles of AD retinas were identified, displaying greater overlap with the temporal cortices than with hippocampi or cerebella. AD retinas exhibited upregulated inflammatory and neurodegenerative processes and downregulated oxidative-phosphorylation/mitochondrial, and photoreceptor-related pathways. This study identifies and maps AD retinopathy, demonstrating the quantitative relationship with brain pathology and cognition.
HostingRepository	PRIDE
AnnounceDate	2025-10-22
AnnouncementXML	Submission_2025-10-21_20:45:07.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mehdi Mirzaei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; carbamoylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-17 02:24:45	ID requested
⏵ 1	2025-10-21 20:45:08	announced

Koronyo Y, Rentsendorj A, Mirzaei N, Regis GC, Sheyn J, Shi H, Barron E, Cook-Wiens G, Rodriguez AR, Medeiros R, Paulo JA, Gupta VB, Kramerov AA, Ljubimov AV, Van Eyk JE, Graham SL, Gupta VK, Ringman JM, Hinton DR, Miller CA, Black KL, Cattaneo A, Meli G, Mirzaei M, Fuchs DT, Koronyo-Hamaoui M, Retinal pathological features and proteome signatures of Alzheimer's disease. Acta Neuropathol, 145(4):409-438(2023) [pubmed]

10.1007/s00401-023-02548-2;

submitter keyword: proteomics, brain, Alzheimer's disease,Retina

Maya Koronyo
contact affiliation	Cedars-Sinai, Neurosurgery - Pavilion, 127 S San Vicente Blvd #A6600, Los Angeles, CA, 90048
contact email	Maya.Koronyo@cshs.org
lab head
Mehdi Mirzaei
contact affiliation	macquarie university
contact email	mehdi.mirzaei@mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD040225

PRIDE project URI

• PRIDE
  1. PXD040225
     1. Label: PRIDE project
     2. Name: Retinal Pathological Features and Proteome Signatures of Alzheimer’s Disease