PXD040207-1

PXD040207 is an original dataset announced via ProteomeXchange.

Title	Cell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis
Description	Deciphering the mechanism of secondary cell wall/SCW formation in vascular plants is key to understanding their development and the molecular basis of biomass recalcitrance. A sophisticated network of transcription factors has been implicated in SCW synthesis in plants, but little is known about the implication of RNA-binding proteins in this process. Here we report that two RNA-binding proteins homologous to the animal translational regulator Musashi, Musashi-Like2/MSIL2 and Musashi-Like4/MSIL4, function redundantly to control SCW formation in interfascicular fibers and the setting of biomass recalcitrance. We show that the disruption of MSIL2/4 decreases the abundance of lignin in fibers and triggers an hypermethylation of glucuronoxylan that is linked to an over-accumulation of GlucuronoXylan Methyltransferase1/3 (GXM1/3) proteins. We demonstrate that MSIL4 binds to the GXM1/3 mRNAs, likely repressing their translation in wild-type plants. Our results reveal cell-type-specific mechanisms underlying SCW formation in Arabidopsis and point to a novel aspect of SCW regulation linking translational repression to regulation of glucuronoxylan methylation.
HostingRepository	PRIDE
AnnounceDate	2023-09-28
AnnouncementXML	Submission_2023-09-28_09:10:25.457.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040207
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	LaurianeKuhn
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 14:54:02	ID requested
⏵ 1	2023-09-28 09:10:25	announced

Dataset with its publication pending

submitter keyword: Arabidopsis, Musashi-type transcriptional regulator,cell wall, interactomic

DominiqueGagliardi
contact affiliation	Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, Strasbourg, France
contact email	dominique.gagliardi@ibmp-cnrs.unistra.fr
lab head
LaurianeKuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040207
     1. Label: PRIDE project
     2. Name: Cell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis