PXD040199-2

PXD040199 is an original dataset announced via ProteomeXchange.

Title	TUBA1C: a new potential target of LncRNA EGFR-AS1 promotes gastric cancer progression
Description	Background: The lack of obvious symptoms of early gastric cancer (GC) as well as the absence of sensitive and specific biomarkers results in poor clinical outcomes. Tubulin is currently emerging as important regulators of the microtubule cytoskeleton and thus have a strong potential to be implicated in a number of disorders, however, its mechanism of action in gastric cancer is still unclear. Tubulin alpha-1C(TUBA1C) is a subtype of α-tubulin, high TUBA1C expression has been shown to be closely related to a poor prognosis in in various cancers,this study, for the first time, revealed the mechanism of TUBA1C promotes malignant progression of gastric cancer in vitro and in vivo. Methods: The expression of lncRNA EGFR-AS1 was detected in human GC cell lines by qRT–PCR. Mass spectrometry experiments following RNA pulldown assays found that EGFR-AS1 directly binds to TUBA1C, the CCK8, EdU, transwell, wound-healing, cell cycle assays and animal experiments were conducted to investigate the function of TUBA1C in GC. Combined with bioinformatics analyses, reveal interaction between Ki-67, E2F1, PCNA and TUBA1C by western blot. Rescue experiments furtherly demonstrated the relationship of EGFR-AS1and TUBA1C. Results: TUBA1C was proved to be a direct target of EGFR-AS1, TUBA1C promotes gastric cancer proliferation, migration and invasion by accelerating the progression of the cell cycle from the G1 phase to the S phase and activating the expression of oncogenes: Ki-67,E2F1 and PCNA. Conclusions: TUBA1C is a new potential target of LncRNA EGFR-AS1 promotes gastric cancer progression and could be a novel biomarker and therapeutic target for GC.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:15:28.627.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lipan peng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 14:23:04	ID requested
1	2023-05-10 14:14:46	announced
⏵ 2	2023-11-14 07:15:29	announced	2023-11-14: Updated project metadata.

Wang H, Cui H, Yang X, Peng L, TUBA1C: a new potential target of LncRNA EGFR-AS1 promotes gastric cancer progression. BMC Cancer, 23(1):258(2023) [pubmed]

submitter keyword: LncRNA EGFR-AS1, TUBA1C,Gastric cancer, Cell cycle

Lipan Peng
contact affiliation	Shandong Provincial Hospital.
contact email	plp1983@126.com
lab head
lipan peng
contact affiliation	Shandong Provincial Hospital
contact email	plp1983@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040199
     1. Label: PRIDE project
     2. Name: TUBA1C: a new potential target of LncRNA EGFR-AS1 promotes gastric cancer progression