PXD040174-1

PXD040174 is an original dataset announced via ProteomeXchange.

Title	Purification of Human β and γ Actin from Budding Yeast
Description	To facilitate biochemical studies of human cytoplasmic actins, Saccharomyces cerevisiaestrains were developed that express either human β-actin or γ-actin as their sole actin. Actins purified from these strains have biochemical properties similar to mammalian skeletal muscle α-actin, but display differential binding to some key actin regulators. Mass-spectrometry analysis shows that the human actins made from yeast generally retain their N-terminal methionines, which are modified by acetylation.
HostingRepository	PRIDE
AnnounceDate	2023-04-24
AnnouncementXML	Submission_2023-04-24_08:35:44.212.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040174
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ebbingde Jong
SpeciesList	scientific name: Oryctolagus sp. 'Rabbit_OD'; NCBI TaxID: 2665958; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 10:06:01	ID requested
⏵ 1	2023-04-24 08:35:45	announced
2	2023-11-14 06:59:27	announced	2023-11-14: Updated project metadata.

10.6019/PXD040174;

Haarer BK, Pimm ML, de Jong EP, Amberg DC, Henty-Ridilla JL, -actin from budding yeast. J Cell Sci, 136(9):(2023) [pubmed]

submitter keyword: β-actin,cytoplasmic actin, non-muscle actin, γ-actin

JessicaHenty-Ridilla
contact affiliation	Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA
contact email	ridillaj@upstate.edu
lab head
Ebbingde Jong
contact affiliation	SUNY Upstate Medical University
contact email	dejonge@upstate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD040174

PRIDE project URI

• PRIDE
  1. PXD040174
     1. Label: PRIDE project
     2. Name: Purification of Human β and γ Actin from Budding Yeast