PXD040156-1

PXD040156 is an original dataset announced via ProteomeXchange.

Title	Deciphering the interaction between TopBP1 and GINS using crosslinking mass spectrometry
Description	Recombinant TopBP1 and GINS were cross linked using the MS non-cleavable crosslinker BS3. Crosslinked proteins were then subsequently either digested directly using the SP3 strategy or first seperated on SDS-PAGE and then in-gel digested. cross-linked peptides were identified by LC-MS. The information from this experiment was used to refine cryo EM structures.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_01:06:40.860.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 08:00:32	ID requested
⏵ 1	2024-05-24 01:06:41	announced

10.1038/s41467-024-45946-0;

Day M, Tetik B, Parlak M, Almeida-Hern, á, ndez Y, R, ä, schle M, Kaschani F, Siegert H, Marko A, Sanchez-Garcia E, Kaiser M, Barker IA, Pearl LH, Oliver AW, Boos D, TopBP1 utilises a bipartite GINS binding mode to support genome replication. Nat Commun, 15(1):1797(2024) [pubmed]

submitter keyword: cross linking, GINS,TopBP1, BS3

Dr Farnusch Kaschani
contact affiliation	Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD040156

PRIDE project URI

• PRIDE
  1. PXD040156
     1. Label: PRIDE project
     2. Name: Deciphering the interaction between TopBP1 and GINS using crosslinking mass spectrometry