PXD040150-1

PXD040150 is an original dataset announced via ProteomeXchange.

Title	Using L3MBTL3 knockout and rescued stable human breast cancer cell line (MDA-MB-231) to detect the novel interacting proteins of L3MBTL3 by immunoaffinity purification and mass spectrometry. Identification of L3MBTL3 interaction proteins in the breast cancer cell.
Description	We used L3MBTL3 knockout MDA-MB-231 cell (L3-KO5+V) and L3MBTL3 rescue MDA-MB-231 cell (L3-KO5+L3). The cells were collected by centrifugation and lysed in lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.1% NP40, 8% glycerol, protease inhibitor cocktail (#P1010, Beyotime, China) on ice for 30 min. Then the cell lysate supernatant was incubated with M2-conjugate agarose beads (#A2220, Merck, USA) by rotation overnight at 4°C. After washing, the beads were eluted by 3×FLAG peptide (#F4799, Merck, USA). Then the elution protein was verified using silver staining, then the target lane was excised and subjected to analyze by an EASY-nLC 1200 UHPLC system (ThermoFisher Scientific, USA) coupled to a Q Exactive HF-X mass spectrometer (ThermoFisher Scientific, USA).
HostingRepository	PRIDE
AnnounceDate	2024-10-25
AnnouncementXML	Submission_2024-10-24_21:02:42.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 07:56:49	ID requested
⏵ 1	2024-10-24 21:02:42	announced

Dataset with its publication pending

submitter keyword: Human, breast cancer cell, L3MBTL3

Jing Li
contact affiliation	Department of Laboratory Medicine & Central Laboratory, Southern Medical University Affiliated Fengxian Hospital, China.
contact email	lijing228nanfang@smu.edu.cn
lab head
Jing Li
contact affiliation	Southern Medical University Affiliated Fengxian Hospital
contact email	lijing228nanfang@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040150
     1. Label: PRIDE project
     2. Name: Using L3MBTL3 knockout and rescued stable human breast cancer cell line (MDA-MB-231) to detect the novel interacting proteins of L3MBTL3 by immunoaffinity purification and mass spectrometry. Identification of L3MBTL3 interaction proteins in the breast cancer cell.