PXD040150 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Using L3MBTL3 knockout and rescued stable human breast cancer cell line (MDA-MB-231) to detect the novel interacting proteins of L3MBTL3 by immunoaffinity purification and mass spectrometry. Identification of L3MBTL3 interaction proteins in the breast cancer cell. |
Description | We used L3MBTL3 knockout MDA-MB-231 cell (L3-KO5+V) and L3MBTL3 rescue MDA-MB-231 cell (L3-KO5+L3). The cells were collected by centrifugation and lysed in lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.1% NP40, 8% glycerol, protease inhibitor cocktail (#P1010, Beyotime, China) on ice for 30 min. Then the cell lysate supernatant was incubated with M2-conjugate agarose beads (#A2220, Merck, USA) by rotation overnight at 4°C. After washing, the beads were eluted by 3×FLAG peptide (#F4799, Merck, USA). Then the elution protein was verified using silver staining, then the target lane was excised and subjected to analyze by an EASY-nLC 1200 UHPLC system (ThermoFisher Scientific, USA) coupled to a Q Exactive HF-X mass spectrometer (ThermoFisher Scientific, USA). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-25 |
AnnouncementXML | Submission_2024-10-24_21:02:42.107.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jing Li |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-16 07:56:49 | ID requested | |
⏵ 1 | 2024-10-24 21:02:42 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, breast cancer cell, L3MBTL3 |
Contact List
Jing Li |
contact affiliation | Department of Laboratory Medicine & Central Laboratory, Southern Medical University Affiliated Fengxian Hospital, China. |
contact email | lijing228nanfang@smu.edu.cn |
lab head | |
Jing Li |
contact affiliation | Southern Medical University Affiliated Fengxian Hospital |
contact email | lijing228nanfang@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040150
- Label: PRIDE project
- Name: Using L3MBTL3 knockout and rescued stable human breast cancer cell line (MDA-MB-231) to detect the novel interacting proteins of L3MBTL3 by immunoaffinity purification and mass spectrometry. Identification of L3MBTL3 interaction proteins in the breast cancer cell.