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PXD040087-1

PXD040087 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleXL-MS of 5S RNP preribosomal complexes
DescriptionThe 5S RNP is assembled from its three components (5S rRNA, Rpl5/uL18, and Rpl11/uL5) before being incorporated into the pre-60S subunit. However, when ribosome synthesis is disturbed, a free 5S RNP can enter the MDM2–p53 pathway to regulate cell cycle and apoptotic signaling. Here we reconstitute and determine the cryo-EM structure of the conserved hexameric 5S RNP with fungal or human factors. This reveals how the nascent 5S rRNA associates with the initial nuclear import complex Syo1–uL18–uL5, and upon further recruitment of two nucleolar factors Rpf2–Rrs1 develops into the 5S RNP precursor that can assemble into the pre-ribosome. In addition, we elucidate the structure of another 5S RNP intermediate, carrying the human ubiquitin ligase Mdm2, which unraveled how this enzyme can be sequestered from its target substrate p53. Our data provide molecular insight into how the 5S RNP can mediate between ribosome biogenesis and cell proliferation.
HostingRepositoryPRIDE
AnnounceDate2023-06-13
AnnouncementXMLSubmission_2023-06-13_08:16:47.294.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHenrikHammarén
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-13 01:27:35ID requested
12023-06-13 08:16:49announced
22023-11-14 09:00:41announced2023-11-14: Updated project metadata.
32024-10-22 05:48:02announced2024-10-22: Updated project metadata.
Publication List
Castillo Duque de Estrada NM, Thoms M, Flemming D, Hammaren HM, Buschauer R, Ameismeier M, Ba, ß, ler J, Beck M, Beckmann R, Hurt E, Structure of nascent 5S RNPs at the crossroad between ribosome assembly and MDM2-p53 pathways. Nat Struct Mol Biol, 30(8):1119-1131(2023) [pubmed]
Keyword List
submitter keyword: 5S RNP, cryo-EM, MDM2
Contact List
MartinBeck
contact affiliationEuropean Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany
contact emailMartin.Beck@biophys.mpg.de
lab head
HenrikHammarén
contact affiliationGenome Biology Unit and Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
contact emailhenrik.hammaren@embl.de
dataset submitter
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Dataset FTP location
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