PXD040033-3

PXD040033 is an original dataset announced via ProteomeXchange.

Title	Peptidomic analysis reveals seasonal neuropeptide and peptide hormone changes in the hypothalamus and pituitary of a hibernating mammal
Description	During the winter, hibernating mammals undergo extreme changes in physiology which allow them to survive without access to food. These animals enter a state of torpor, which is characterized by a decreased metabolism, near-freezing body temperatures, and a dramatically reduced heart rate. The neurochemical basis of this regulation is largely unknown. Based on prior evidence suggesting that the peptide-rich hypothalamus plays critical roles in hibernation, we hypothesized that changes in specific cell-cell signaling peptides (neuropeptides and peptide hormones) underlie physiological changes during torpor/arousal cycles. To test this hypothesis, we used a mass spectrometry-based peptidomics approach to examine seasonal changes of endogenous peptides that occur in the hypothalamus and pituitary of a model hibernating mammal, the thirteen-lined ground squirrel (Ictidomys tridecemlineatus). In the pituitary, we observed changes in a number of distinct peptide hormones as animals prepare for torpor in October, exit torpor in March, and progress from Spring (March) to Fall (August). In the hypothalamus, we observed an overall increase in neuropeptides in October (pre-torpor), a decrease as the animal enters torpor, and an increase in a subset of neuropeptides during normothermic interbout arousals. Notable changes were observed for feeding regulatory peptides from NPY and proSAAS prohormones, opioid peptides from PENK, PDYN, and POMC prohormones, and a number of peptides without well-established functions. In contrast to transcriptome and antibody-based measurements, our mass spectrometry-based approach allowed the identification and measurement of the final processed forms of these peptides after extensive post-translational modifications. Overall, our study provides critical insight into changes in endogenous peptides in the hypothalamus and pituitary during mammalian hibernation that were not available from transcriptome measurements. Understanding the molecular basis underlying the hibernation phenotype may pave the way for future efforts to employ hibernation-like strategies for organ preservation, combating obesity, and treatments for stroke.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:52:39.265.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040033
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Checco
SpeciesList	scientific name: Spermophilus tridecemlineatus (Thirteen-lined ground squirrel); NCBI TaxID: 43179;
ModificationList	2-pyrrolidone-5-carboxylic acid; amidated residue; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Xevo G2-S QTof

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-10 06:22:01	ID requested
1	2023-07-10 08:06:32	announced
2	2023-11-14 07:05:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:52:39	announced	2024-10-22: Updated project metadata.

10.1021/ACSCHEMNEURO.3C00268;

10.6019/PXD040033;

submitter keyword: peptidomics, hypothalamus, pituitary, neuropeptides,Hibernation, peptide hormones

James Checco
contact affiliation	Department of Chemistry University of Nebraska-Lincoln Lincoln, NE 68588 USA
contact email	checco@unl.edu
lab head
James Checco
contact affiliation	University of Nebraska-Lincoln
contact email	checco@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD040033

PRIDE project URI

• PRIDE
  1. PXD040033
     1. Label: PRIDE project
     2. Name: Peptidomic analysis reveals seasonal neuropeptide and peptide hormone changes in the hypothalamus and pituitary of a hibernating mammal