PXD040024-1

PXD040024 is an original dataset announced via ProteomeXchange.

Title	Bipartite GINS binding mode of TopBP1 to activate the replicative helicase - Chromass Data Set
Description	How the replicative Mcm2-7 helicase is activated during replication origin firing remains largely unknown. Our biochemical and structural studies reveal that the helicase activator GINS interacts with TopBP1 using two separate binding surfaces. One involves a stretch of highly conserved amino acids in the TopBP1-GINI domain. The other surface is located in TopBP1-BRCT4. The two surfaces bind to opposite ends of the A domain of the GINS subunit Psf1, and their cooperation is required for a biochemically stable TopBP1-GINS interaction. The GINI and BRCT4 domains also cooperate during replication origin firing, as immune-replacement experiments in Xenopus egg extract using different GINI and BRCT4 mutations suggest. The TopBP1-GINS interaction is incompatible with simultaneous binding of DNA polymerase epsilon to GINS when bound to Mcm2-7-Cdc45. Our TopBP1-GINS model predicts the coordination of three molecular processes, DNA polymerase epsilon arrival, TopBP1 ejection and GINS integration into Mcm2-7-Cdc45.
HostingRepository	PRIDE
AnnounceDate	2024-03-26
AnnouncementXML	Submission_2024-03-26_01:03:10.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-10 04:57:22	ID requested
⏵ 1	2024-03-26 01:03:10	announced
2	2024-10-22 06:34:35	announced	2024-10-22: Updated project metadata.

10.1038/S41467-024-45946-0;

submitter keyword: Xenopus egg extracts, DNA replication

Markus Räschle
contact affiliation	Molecular Genetics, RPTU Kaiserslautern
contact email	raeschle@bio.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040024
     1. Label: PRIDE project
     2. Name: Bipartite GINS binding mode of TopBP1 to activate the replicative helicase - Chromass Data Set