PXD040000-2

PXD040000 is an original dataset announced via ProteomeXchange.

Title	Confirmation of replication interactome of TopBP1 using proximity-dependent biotinylation MS
Description	Goal of the Experiment is to confirm the replication interactome of TopBP1 using stabile 293 FlpIn cell lines expressing TopBP1 adapter fused to APEX2. Proteins in its close proximity will become biotinylated by APEX2, thereby enabling the pulldown of biotinylated proteins and thus, replication involved substrates of TopBP1
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:40:39.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-09 01:56:26	ID requested
1	2024-05-21 12:22:41	announced
⏵ 2	2024-10-22 06:40:40	announced	2024-10-22: Updated project metadata.

10.1038/s41467-024-45946-0;

Day M, Tetik B, Parlak M, Almeida-Hern, á, ndez Y, R, ä, schle M, Kaschani F, Siegert H, Marko A, Sanchez-Garcia E, Kaiser M, Barker IA, Pearl LH, Oliver AW, Boos D, TopBP1 utilises a bipartite GINS binding mode to support genome replication. Nat Commun, 15(1):1797(2024) [pubmed]

submitter keyword: TopBP1,proximity-dependent biotinylation

Dr Farnusch Kaschani
contact affiliation	Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD040000

PRIDE project URI

• PRIDE
  1. PXD040000
     1. Label: PRIDE project
     2. Name: Confirmation of replication interactome of TopBP1 using proximity-dependent biotinylation MS