PXD039979-1

PXD039979 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Immunoprecipitation mass spectrometry of SPARKEL
Description	SPARK is an AGC kinase found in apicomplexan parasites, which are ubiquitous pathogens of animals. Previous studies have shown that SPARK is necessary for efficient parasite calcium mobilization, invasion, and egress from host cells. We identified an interaction partner of SPARK, which we termed SPARKEL. Here, we performed immunoprecipitation of SPARKEL from parasite lysates followed by mass spectrometry to confirm the interaction with SPARK and identify other potential interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:19:15.688.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039979
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alice Herneisen
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	methylthiolated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-09 00:56:02	ID requested
⏵ 1	2024-10-08 11:19:16	announced
2	2024-10-22 06:49:33	announced	2024-10-22: Updated project metadata.

Publication List

10.6019/PXD039979;
10.7554/ELIFE.93877.1;

10.6019/PXD039979;

10.7554/ELIFE.93877.1;

Keyword List

submitter keyword: Toxoplasma gondii, SPARKEL

submitter keyword: Toxoplasma gondii, SPARKEL

Contact List

Sebastian Lourido
contact affiliation	Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology
contact email	lourido@wi.mit.edu
lab head
Alice Herneisen
contact affiliation	Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology
contact email	alice@wi.mit.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD039979
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD039979

PRIDE project URI

Repository Record List

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