PXD039943-2

PXD039943 is an original dataset announced via ProteomeXchange.

Title	The effects of silencing PCYOX1 on the proteome of murine fibroblast cells 3t3-L1 differentiated to adipocyte-like cells
Description	In order to evaluate the impact of PCYOX1 deletion on adipogenesis, we applied a label-free mass spectrometry-based proteomic approach to compare the proteome of 3T3-L1 differentiated for 9 days to adipocytes after PCYOX1 gene silencing. Pcyox1 was silenced using shRNA lentiviral particles. Control cells were treated with shRNA lentiviral particles containing a negative construct.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:59.094.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039943
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Erica Gianazza
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-08 02:02:28	ID requested
1	2023-05-10 09:33:44	announced
⏵ 2	2023-11-14 08:45:59	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:45:23	announced	2024-10-22: Updated project metadata.

10.6019/PXD039943;

Banfi C, Mallia A, Ghilardi S, Brioschi M, Gianazza E, Eligini S, Sahl, é, n P, Baetta R, Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis. Antioxidants (Basel), 12(3):(2023) [pubmed]

submitter keyword: 3T3-L1 cells, label-free mass spectrometry,PCYOX1

Cristina Banfi
contact affiliation	Proteomics and Metabolomics Unit, Monzino Cardiologic Center, IRCCS, Milan, Italy
contact email	cristina.banfi@cardiologicomonzino.it
lab head
Erica Gianazza
contact affiliation	Centro Cardiologico Monzino
contact email	erica.gianazza@cardiologicomonzino.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039943

PRIDE project URI

• PRIDE
  1. PXD039943
     1. Label: PRIDE project
     2. Name: The effects of silencing PCYOX1 on the proteome of murine fibroblast cells 3t3-L1 differentiated to adipocyte-like cells