PXD039934-2

PXD039934 is an original dataset announced via ProteomeXchange.

Title	Changes in glycosylated proteins in colostrum and mature milk and their implication
Description	Glycosylation is one of the essential post-translational modifications that influences the function of milk proteins. In the present study, 998 proteins and 764 glycosylated sites from 402 glycoproteins were identified in human milk by TMT labeling proteomics. Compared to human milk proteins, the glycoproteins were mainly enriched in cell adhesion, proteolysis and defense/immune process. The abundance of 353 glycosylated sites and their 179 parent proteins was quantified. After normalization to their parent protein's abundance, 78 glycosylated sites in 56 glycoproteins and 10 glycosylated sites in 10 glycoproteins were significantly higher in colostrum and mature milk, respectively. These changed glycoproteins were mainly related to host defense. Intriguingly, one glycosylated site (Asp144) in IgA and two glycosylated sites (Asp38 and Asp1079) in Tenascin are significantly upregulated even though their protein abundance was downregulated during lactation. This study helps us figure out the critical glycosylated sites in proteins that might influence their biological function in an unbiased way.
HostingRepository	iProX
AnnounceDate	2023-02-08
AnnouncementXML	Submission_2023-07-03_20:46:46.758.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Lu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	TMT6plex reporter fragment
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-07 21:59:02	ID requested
1	2023-02-07 21:59:21	announced
⏵ 2	2023-07-03 20:46:47	announced	2023-07-04: Update publication information.

Lu J, Zhang W, Ma C, Pang X, Dai Y, Zhu T, Liu J, Xing L, Zhang S, Lv J, Changes in glycosylated proteins in colostrum and mature milk and their implication. Front Nutr, 10():1161310(2023) [pubmed]

submitter keyword: glycoproteins, colostrum, mature milk, host defense, IgA

Jing Lu
contact affiliation	Beijing Technology and Business University
contact email	lujing@bubu.edu.cn
lab head
Jing Lu
contact affiliation	Beijing Technology and Business University
contact email	wangxinyu@ibp.ac.cn
dataset submitter

iProX dataset URI