PXD039931-2

PXD039931 is an original dataset announced via ProteomeXchange.

Title	Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows
Description	Macrocyclic peptides are attractive for chemoproteomic applications due to their modular synthesis and potential for high target selectivity. We describe a solid phase synthesis method for the efficient generation of libraries of small macrocycles that contain an electrophile and alkyne handle. The modular synthesis produces libraries that can be directly screened using simple SDS-PAGE readouts and then optimal lead molecules applied to proteomic analysis. We generated a library of 480 macrocyclic peptides containing the weakly reactive fluorosulfate (OSF) electrophile. Initial screening of a subset of the library containing each of the various diversity elements identified initial molecules of interest. The corresponding positional and confirmational isomers were then screened to select molecules that showed specific protein labeling patterns that were dependent on the probe structure. The most promising hits were applied to standard chemoproteomic workflows to identify protein targets. Our results demonstrate the feasibility of rapid, on-resin synthesis of diverse macrocyclic electrophiles to generate new classes of covalent ligands.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:27.204.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Abegg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-07 14:28:21	ID requested
1	2023-03-02 16:11:57	announced
⏵ 2	2023-11-14 08:24:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: SuFEx, chemoproteomics, electrophile, macrocycles,Fluorosulfate

Dr. Alexander Adibekian
contact affiliation	Department of Chemistry University of Illinois at Chicago 845 West Taylor Street - Room 1454 SES Chicago, Illinois, 60607, USA
contact email	aadibeki@uic.edu
lab head
Daniel Abegg
contact affiliation	University of Illinois at Chicago
contact email	dabegg@uic.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD039931

PRIDE project URI

• PRIDE
  1. PXD039931
     1. Label: PRIDE project
     2. Name: Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows