PXD039904-1

PXD039904 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Octanedioic-acid supplementation protects from acute kidney injury via stimulation of renal peroxisomal activity over mitochondrial
Description	Lysine succinylation is a posttranslational modification associated with the control of several diseases, including acute kidney injury (AKI). Hypersuccinylation favors peroxisomal fatty acid oxidation (FAO) instead of mitochondrial. In addition, the medium-chain fatty acids (MCFAs) dodecanedioic acid (DC12) and octanedioic acid (DC8), upon FAO, generate succinyl-CoA, resulting in hypersuccinylation. To test the protective roles of MCFAs during AKI, mice were fed with control, 10% DC12, or 10% DC8 diet, then, subjected to either ischemic-AKI , or cisplatin-AKI models. Supplementation was provided until sacrifice. Biochemical, histologic, genetic, and proteomic analysis were performed, the latter involving a lysine-succinylome-based analysis. Both DC8 and DC12 prevented the rise of AKI markers in mice that underwent renal injury. However, DC8 was even more protective against AKI than DC12. Finally, succinylome analysis evidenced that the kidneys of DC8-fed mice showed an extensive succinylation of peroxisomal activity-related proteins, and a decline in mitochondrial FAO, in comparison to control-fed mice. DC8 supplementation drives renal protein hypersuccinylation, promoting a shift from mitochondrial to peroxisomal FAO, and protecting against AKI. DC8 is convenient, inexpensive, easily administered, and efficient. We believe this study could be translated in the future to clinical settings, which would highly benefit patients at high risk of AKI.
HostingRepository	MassIVE
AnnounceDate	2023-10-24
AnnouncementXML	Submission_2023-10-24_11:44:54.990.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joanna Bons
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	N6-succinyl-L-lysine
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-06 18:12:40	ID requested
⏵ 1	2023-10-24 11:44:55	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Data-independent acquisition (DIA), Post-translational modifications, Succinylation, Acute kidney injury , Fatty acid oxidation, Octanedioic-acid supplementation

submitter keyword: Data-independent acquisition (DIA), Post-translational modifications, Succinylation, Acute kidney injury , Fatty acid oxidation, Octanedioic-acid supplementation

Contact List

Birgit Schilling
contact affiliation	Buck Institute
contact email	bschilling@buckinstitute.org
lab head
Joanna Bons
contact affiliation	Buck Institute for Research on Aging
contact email	jbons@buckinstitute.org
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091214/