⮝ Full datasets listing
PXD039902-1
PXD039902 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Differential proteome profiling of bacterial culture supernatants reveals candidates for the induction of oral immune priming in the Red Flour Beetle |
| Description | Most organisms are hosts to a wide range of symbionts and commensals, but they might also encounter parasites and pathogens during their lifetime. Therefore, immune strategies to recognize and counteract potential threats have evolved across all kingdoms of life. The most elaborate immune defences are found in vertebrate animals, where responses are classically divided into innate and adaptive. Insects lack the cellular machinery needed to provide adaptive immunity. However, over the past decades, work on immune priming has shown that to a certain degree, insects are indeed capable of specifically recognizing, memorizing, and counteracting certain pathogens. An important piece of the puzzle are the pathogen-associated molecules that induce these immune priming responses. Here, we make use of the model system Tribolium castaneum (T. castaneum) and its pathogen Bacillus thuringiensis (B. thuringiensis), to compare the proteomes of two closely related B. thuringiensis strains that either induce priming via the oral route, or not. We identify proteins that might be immunostimulatory in T. castaneum. The Cry3Aa toxin, an important plasmid-encoded virulence factor of Bacillus thuringiensis bv. tenebrionis (hereafter called Btt) was found to be one of the most promising candidates. We made use of additional B. thuringiensis strains varying in the possession of the Cry-carrying plasmid to experimentally test for its relevance for priming. Our findings will help future studies to focus on specific candidates to perform experiments on the mechanisms and evolution of immune priming. |
| HostingRepository | jPOST |
| AnnounceDate | 2023-11-01 |
| AnnouncementXML | Submission_2025-10-24_00:33:23.489.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jürgen Eirich |
| SpeciesList | scientific name: Bacillus thuringiensis serovar thuringiensis; NCBI TaxID: 1432; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-02-06 08:22:01 | ID requested | |
| ⏵ 1 | 2025-10-24 00:33:24 | announced |
Publication List
| L, ä, nger ZM, Baur M, Kor, š, a A, Eirich J, Lindeza AS, Zanchi C, Finkemeier I, Kurtz J, Differential proteome profiling of bacterial culture supernatants reveals candidates for the induction of oral immune priming in the red flour beetle. Biol Lett, 19(11):20230322(2023) [pubmed] |
Keyword List
| submitter keyword: Insects, immune priming, Tribolium castaneum, Bacillus thuringiensis, Cry3Aa, Secretome |
Contact List
| Iris Finkemeier | |
|---|---|
| lab head | |
| Jürgen Eirich | |
| contact affiliation | University of Münster - WWU - Institute for Biology and Biotechnology of Plants |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
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