PXD039892 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of proteins that interact with Siglec-6 |
Description | Siglec-6 is a glycoprotein overexpressed on chronic lymphocytic leukemia (CLL) cell. We have shown that Siglec-6 promotes migration and adhesion of CLL cells towards CLL bone marrow stromal cells. To elucidate the mechanistic pathway involved in Siglec-6 dependent migration, we performed mass spectrometry proteomics analysis on MEC1-002 CLL cell line pulled down with a Siglec-6 targeted antibody, which revealed that Siglec-6 interacts with DOCK8, a guanine nucleotide exchange factor. Interaction of Siglec-6 with its ligand promotes DOCK8 dependent Cdc42 activation, WASP protein recruitment and F-actin polymerization. Therapeutically, Siglec-6 leukemic cells can be eliminated with a Siglec-6 targeted bispecific antibody in vitro and in vivo. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-08 |
AnnouncementXML | Submission_2024-05-08_08:51:10.681.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jessica Nunes |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-06 05:56:32 | ID requested | |
⏵ 1 | 2024-05-08 08:51:11 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, cell migration, chronic lymphocytic leukemia |
Contact List
Natarajan Muthusamy |
contact affiliation | Professor, Associate Director of Academic Affairs, Division of Hematology, Professor of Medicine, Dept of Internal Medicine |
contact email | raj.muthusamy@osumc.edu |
lab head | |
Jessica Nunes |
contact affiliation | Postdoctoral researcher |
contact email | nunes.17@osu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD039892 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039892
- Label: PRIDE project
- Name: Identification of proteins that interact with Siglec-6