PXD039892

PXD039892 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins that interact with Siglec-6
Description	Siglec-6 is a glycoprotein overexpressed on chronic lymphocytic leukemia (CLL) cell. We have shown that Siglec-6 promotes migration and adhesion of CLL cells towards CLL bone marrow stromal cells. To elucidate the mechanistic pathway involved in Siglec-6 dependent migration, we performed mass spectrometry proteomics analysis on MEC1-002 CLL cell line pulled down with a Siglec-6 targeted antibody, which revealed that Siglec-6 interacts with DOCK8, a guanine nucleotide exchange factor. Interaction of Siglec-6 with its ligand promotes DOCK8 dependent Cdc42 activation, WASP protein recruitment and F-actin polymerization. Therapeutically, Siglec-6 leukemic cells can be eliminated with a Siglec-6 targeted bispecific antibody in vitro and in vivo.
HostingRepository	PRIDE
AnnounceDate	2024-05-08
AnnouncementXML	Submission_2024-05-08_08:51:10.681.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Nunes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-06 05:56:32	ID requested
⏵ 1	2024-05-08 08:51:11	announced

Dataset with its publication pending

submitter keyword: Human, cell migration, chronic lymphocytic leukemia

Natarajan Muthusamy
contact affiliation	Professor, Associate Director of Academic Affairs, Division of Hematology, Professor of Medicine, Dept of Internal Medicine
contact email	raj.muthusamy@osumc.edu
lab head
Jessica Nunes
contact affiliation	Postdoctoral researcher
contact email	nunes.17@osu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD039892

PRIDE project URI

• PRIDE
  1. PXD039892
     1. Label: PRIDE project
     2. Name: Identification of proteins that interact with Siglec-6