PXD039871-1

PXD039871 is an original dataset announced via ProteomeXchange.

Title	Multi-omics revealed the difference in epididymal cauda expression in yak (Bos grunniens) before and after sexual maturation
Description	Normal epididymis development is the basis of male reproduction, and epididymis is a crucial site where sperm maturation occurs. Although epididymis has been studied through multiple omics methods, combined omics has not been used to present the differential genes in the epididymal cauda of yaks before and after sexual maturity. We here provided the landscape maps of differential genes and proteins obtained through RNA-seq and proteomics technology, and searched for potential key genes, including TGFBI, COL1A1, COL1A2, COL3A1, COL12A1, SULT2B1, KRT19, and NPC2, through integration analysis. In separate and combined enrichment analyses, genes mainly related to cell growth, differentiation and adhesion, sperm maturation, and immune defense were differentially expressed. The involved pathways included extracellular matrix (ECM)–receptor interaction, protein differentiation and absorption, lysosomes, and estrogen signaling pathway. When the sequencing results were verified through qRT-PCR and 4D-PRM, they were found to be consistent. In addition, after the key genes were identified, abnormal expression of these genes was found to possibly cause the retardation of cauda epididymis development and abnormal sperm function in yaks. In conclusion, we provide useful clues for the development of epididymal cauda of yak, sperm maturation, and screening of key genes involved in reproductive regulation of male yak through separate and combined analyses.
HostingRepository	iProX
AnnounceDate	2023-02-04
AnnouncementXML	Submission_2023-02-05_19:24:29.102.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziqiang Ding
SpeciesList	scientific name: Bos grunniens; NCBI TaxID: 30521;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-05 19:24:15	ID requested
⏵ 1	2023-02-05 19:24:29	announced
2	2023-05-06 00:08:42	announced	2023-05-06: Update publication information.

Dataset with its publication pending

submitter keyword: epididymis, proteomics, RNA-seq, spermioteleosis, yak

Xian Guo
contact affiliation	Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences
contact email	2964758904@qq.com
lab head
Ziqiang Ding
contact affiliation	Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences
contact email	985631441@qq.com
dataset submitter

iProX dataset URI