PXD039863 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Combined transcriptome and proteome analysis of the protein composition of the brochosomes of the leafhopper Nephotettix cincticeps |
Description | Brochosomes are a unique coating on the integuments of Cicadellidae produced in specialized glandular sections of Malpighian tubules. However, knowledge of the protein composition of brochosome is very limited. In this study, transcriptomic and proteomic profiling was performed to characterize the protein composition of the brochosome in rice green leafhopper Nephotettix cincticeps. Brochosomes were collected from the forewings of leafhoppers using ultrasonic treatment allowing for more effective brochosome collection and shock treatment resulting in purer brochosomes . After combined proteomic and transcriptomic analysis, 12 putative major structural proteins of the brochosome (BSM) and 10 brochosome-associated proteins (BSAP) were identified. Each BSM has specific conserved motifs containing several cysteines cross-linked by disulfide bonds. BSAP, which may be loosely linked to the outer and inner surfaces of the brochosomes, might play an important role in the proper folding of the BSM and resisting pathogens. Apart from a few BSAPs associated with cyclase, homologs of BSM and BSAP were present exclusively in the insect family Cicadellidae rather than other Hemiptera species, suggesting that these proteins might be encoded by an orphan gene family specific to the Cicadellidae. This is the first study to identify and characterize the protein composition of the brochosome of N. cincticeps, which will provide a solid foundation for further studies of brochosome synthesis, function, and evolutionary origins. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_07:15:46.202.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | wei wu |
SpeciesList | scientific name: Nephotettix cincticeps (Green rice leafhopper) (Selenocephalus cincticeps); NCBI TaxID: 94400; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-04 13:55:39 | ID requested | |
⏵ 1 | 2024-01-26 07:15:47 | announced | |
Publication List
Keyword List
submitter keyword: Brochosome, Nephotettix cincticeps, Leafhopper, Transcriptome, LC-MS/MS |
Contact List
wei wu |
contact affiliation | State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China |
contact email | wuwei1@nbu.edu.cn |
lab head | |
wei wu |
contact affiliation | +860 187 5016 2795 |
contact email | wuwei1@nbu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039863
- Label: PRIDE project
- Name: Combined transcriptome and proteome analysis of the protein composition of the brochosomes of the leafhopper Nephotettix cincticeps