PXD039764-1

PXD039764 is an original dataset announced via ProteomeXchange.

Title	The Extracellular Vesicles Proteome of Endometrial Cells Simulating the Receptive Menstrual Phase Differs from That of Endometrial Cells Simulating the Non-Receptive Menstrual Phase
Description	Successful embryo implantation into a receptive endometrium requires mutual endometrial-embryo communication. Recently, the function of extracellular vehicles (EVs) in cell-to-cell interaction in embryo-maternal interactions has been investigated. We explored isolated endometrial derived EVs, using RL95-2 cells as a model of a receptive endometrium, influenced by menstrual cycle hormones estrogen (E2; proliferative phase) progesterone (P4; secretory phase) and estrogen plus progesterone (E2P4; the receptive phase). EV sized particles were isolated by differential centrifugation and size exclusion chromatography. Nanoparticle tracking analysis was used to examine the different concentration and size of particles and EV proteomic analysis per-formed using shotgun label-free mass spectrometry. Our results showed that although endome-trial derived EVs were secreted in numbers independent of hormonal stimulation, EVs sizes were statistically modified by it. Proteomics analysis showed that hormone treatment changes affect the endometrial EVs proteome, with proteins enhanced within the EV E2P4 group shown to be in-volved in different processes such as embryo implantation, endometrial receptivity, and embryo development, supporting the concept of a communication system between the embryo and the maternal endometrium via EVs.
HostingRepository	PRIDE
AnnounceDate	2023-02-13
AnnouncementXML	Submission_2023-02-13_02:15:16.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AmberHart
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-01 05:04:26	ID requested
⏵ 1	2023-02-13 02:15:17	announced

Dataset with its publication pending

submitter keyword: hormones,Extracellular vesicels

AlirezaFazeli
contact affiliation	Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabo-lism, The Medical School, University of Sheffield, Sheffield , UK And Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, Faculty of Medi-cine, University of Tartu, 14B Ravila, 50411 Tartu, Estonia and Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences
contact email	a.fazeli@sheffield.ac.uk
lab head
AmberHart
contact affiliation	The university of sheffield
contact email	arhart1@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039764
     1. Label: PRIDE project
     2. Name: The Extracellular Vesicles Proteome of Endometrial Cells Simulating the Receptive Menstrual Phase Differs from That of Endometrial Cells Simulating the Non-Receptive Menstrual Phase