PXD039757-1

PXD039757 is an original dataset announced via ProteomeXchange.

Title	Hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes
Description	Hormonal protocols for cervix dilation are essential for non-surgical embryo recovery (NSER) in ewes, due to the cervical complexity of the species. However, little is known about the possible effects of these hormones on the uterine environment. Thus, this study aimed to evaluate in sheep the effect of a hormonal protocol for cervical dilation on the protein profile of the uterine fluid. After superovulation, Santa Inês ewes (n = 40) were allocated into either a treated group with estradiol benzoate (100 µg, iv) and sodium cloprostenol (0.12 mg, im) 12 h before the embryo collection plus oxytocin (100 IU, iv) 15 min before the collection procedure; or a control group, which consisted of the administration of saline solution. The recovery of the uterine lavage and embryos was performed by laparotomy. After precipitation (trichloroacetic acid) and digestion (trypsin), proteins were identified by mass spectrometry (LC-MS/MS). Overall, 1447 proteins were identified, of which 436 and 126 were found exclusively in the treated and control groups, respectively. A total of 16 proteins were highly expressed in the treated group (P < 0.05), which included glutathione transferase (related to prostaglandin biosynthesis) and osteopontin (involved in the regulation of estradiol). In the control group, five proteins were up-regulated (P < 0.05), including cofilin (linked to up-regulation of embryonic development). Based on the gene ontology, in both groups, the proteins most abundantly in the uterine fluid are related to cellular and metabolic processes, in addition to locomotion and localization in the control group. In conclusion, the hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes after embryo collection.
HostingRepository	PRIDE
AnnounceDate	2025-11-14
AnnouncementXML	Submission_2025-11-13_19:55:10.734.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Unit
SpeciesList	scientific name: Ovis aries; NCBI TaxID: NEWT:9940;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-01 03:53:55	ID requested
⏵ 1	2025-11-13 19:55:11	announced

10.1016/J.RVSC.2025.105706;

submitter keyword: MOET,Proteomics, sheep, embryo collection

Fábio César Sousa Nogueira
contact affiliation	Laboratório Unidade Proteômica, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira Ramos, 149, Rio de Janeiro, Rio de Janeiro, CEP 21941-611, Brazil
contact email	fabiocsn@gmail.com
lab head
Proteomics Unit
contact affiliation	UFRJ
contact email	unidadeproteomica@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039757
     1. Label: PRIDE project
     2. Name: Hormonal protocol for cervical dilation modifies the protein profile of uterine lavage recovered from superovulated ewes