PXD039752-1

PXD039752 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Development of cytosol-facing organelle outer membrane proximity labeling systems in Arabidopsis
Description	The cytosol-facing organelle outer membrane (OM) is a layer that separates from but also communicates with cellular compartments for the import and exchange of proteins, metabolites and signaling molecules. OM resident proteins are also the targets of cellular surveillance systems by which the cell controls organelle homeostasis for promoting cell survival under stress. However, discovery of OM proteins and their dynamically interacting partners by traditional approaches can be very challenging. In this study, we developed an outer membrane proximity labeling system (OMPL) using biotin ligase mediated proximity biotinylation to map the proximity proteome of the cytosol-facing mitochondria, chloroplast and peroxisome outer membranes in living Arabidopsis cells. The power of this optimized system is highlighted by the discovery of the cytosolic factors and OM receptor candidates that are potentially involved in protein local translation and translocation, membrane contact sites and organelle quality control. Moreover, this OMPL plants generated in this study can be used for rapid and specific isolation of intact mitochondria and peroxisome. Our findings provide ample ground for further investigations on important questions in organelle biology.
HostingRepository	jPOST
AnnounceDate	2024-01-31
AnnouncementXML	Submission_2024-01-30_07:00:12.011.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	tian
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; N6-biotinyl-L-lysine
Instrument	Q Exactive HF-X; Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-01 02:00:59	ID requested
⏵ 1	2024-01-30 07:00:12	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Arabidopsis thaliana, Proximity labeling, Biotin Protein Ligase, Organelle outer membrane proximity labeling, Organelle outer membrane proximity proteome, Co-translational protein import, Membrane contact sites, Organelle quality control.

submitter keyword: Arabidopsis thaliana, Proximity labeling, Biotin Protein Ligase, Organelle outer membrane proximity labeling, Organelle outer membrane proximity proteome, Co-translational protein import, Membrane contact sites, Organelle quality control.

Contact List

Xiaohong Zhu
lab head
tian
contact affiliation	Southern University of science and technology
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002016/