PXD039742-1

PXD039742 is an original dataset announced via ProteomeXchange.

Title	Tandem mass tag (TMT)-based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae
Description	In this study, quantitative proteomics tandem mass tag (TMT) labeling technology was used to detect A Halolimnae protein was identified and quantitatively analyzed. Compared with the control group, 127521 and 672 differentially expressed proteins (DEPs) (Fold change ≤ 0.667 or ≥ 1.5, p<0.05) were identified under the treatment of 8%, 12% and 16% NaCl in the experimental group. GO (Gene Ontology) annotation analysis, COG (Clusters of Orthologous Groups of proteins) functional annotation and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotation were used for enrichment analysis of differentially expressed proteins. A Halolimnae adopted different strategies to deal with osmotic stress. The main mechanism of salt adaptation is to synthesize tetrahydropyrimidine and hydroxytetrahydropyrimidine, synthesize and absorb glycine betaine and proline, and accumulate different kinds and concentrations of compatible solutes in the cell. Among them, tetrahydropyrimidine synthesis-related proteins were up-regulated under low salt, high salt and extremely high salt conditions, and the contents of EctA, EctB, EctC and intracellular tetrahydropyrimidine increased significantly with the increase of salinity. Proteins related to the synthesis and transport of compatible solutes such as hydroxytetrahydropyrimidine, glycine betaine, proline, glutamine and glutamic acid were also up-regulated under different salinity conditions. At high salt concentration, cell metabolism slows down, but amino acid metabolism, carbohydrate metabolism and membrane transport related to salt adaptation are significantly regulated, providing energy and precursors for the accumulation of compatible solutes, and maintaining the balance of intracellular and extracellular permeability. In addition, the protein associated with Na+/K+ion transport seems to be in A Halolimnae played little role in the adaptation process of long-term osmotic stress.
HostingRepository	iProX
AnnounceDate	2023-01-30
AnnouncementXML	Submission_2023-06-01_18:56:40.198.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qinghua Xing
SpeciesList	scientific name: Alkalicoccus halolimnae; NCBI TaxID: 1667239;
ModificationList	TMT6plex reporter fragment
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-30 18:12:55	ID requested
⏵ 1	2023-06-01 18:56:40	announced
2	2024-02-03 20:37:00	announced	2024-02-04: Update publication information.

Dataset with its publication pending

submitter keyword: Moderate halophile, Alkalicoccus halolimnae, TMT-proteomics

Baisuo Zhao
contact affiliation	Graduate School, Chinese Academy of Agricultural Sciences
contact email	bszhao@163.com
lab head
Qinghua Xing
contact affiliation	Graduate School, Chinese Academy of Agricultural Sciences
contact email	xingqinghua7777@163.com
dataset submitter

iProX dataset URI