PXD039719 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | EED and IgG (as control) co-immunoprecipitation of wild type embryonic stem cells (ESCs), with 3 biological replicates per condition. |
Description | DNA and Histone-3 Lysine 27 methylation typically function as repressive modifications and operate within distinct genomic compartments. In mammals, the majority of the genome is kept in a DNA methylated state, whereas the Polycomb Repressive Complexes regulate the unmethylated CpG-rich promoters of developmental genes. In contrast to this general framework, the extraembryonic lineages display non-canonical, globally intermediate DNA methylation levels that includes disruption of local Polycomb domains. To better understand this unusual landscape’s molecular properties, we genetically and chemically perturbed major epigenetic pathways in mouse Trophoblast Stem Cells (TSCs). We find that the extraembryonic epigenome reflects ongoing and dynamic de novo methyltransferase recruitment, which is continuously antagonized by Polycomb to maintain intermediate, locally disordered methylation. Despite its disorganized molecular appearance, our data point to a highly controlled equilibrium between counteracting repressors within extraembryonic cells, one that can seemingly persist indefinitely without bistable features typically seen for embryonic forms of epigenetic regulation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-02-06 |
AnnouncementXML | Submission_2023-02-06_01:35:16.826.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | DavidMeierhofer |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | timsTOF SCP |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-27 10:53:47 | ID requested | |
⏵ 1 | 2023-02-06 01:35:17 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: epigenetic regulation,mouse embryonic stem cells, EED, Polycomb |
Contact List
AlexanderMeissner |
contact affiliation | Department of Genome Regulation, Max Planck Institute for Molecular Genetics; Berlin, Germany |
contact email | meissner@molgen.mpg.de |
lab head | |
DavidMeierhofer |
contact affiliation | Mass Spectrometry Facility MPIMG |
contact email | meierhof@molgen.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039719 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039719
- Label: PRIDE project
- Name: EED and IgG (as control) co-immunoprecipitation of wild type embryonic stem cells (ESCs), with 3 biological replicates per condition.