PXD039679-1

PXD039679 is an original dataset announced via ProteomeXchange.

Title	Proteomic analyses identified distinct types of vesicles essential for egress of Plasmodium falciparum gametocytes from red blood cells
Description	Transmission of malaria parasites to the mosquito is mediated by sexual precursor cells, the gametocytes. Upon entering the mosquito midgut, the gametocytes egress from the enveloping erythrocyte while passing through gametogenesis. Egress follows an inside-out mode during which the membrane of the parasitophorous vacuole ruptures prior to the erythrocyte membrane. Membrane rupture requires the exocytosis of specialized secretory vesicles of the parasites; i.e. the osmiophilic bodies (OBs) involved in rupture of the parasitophorous vacuole membrane, and vesicles (here termed g-exonemes) that harbour the perforin-like protein PPLP2 needed for erythrocyte lysis. While several OB proteins are known, like G377 and MDV1/Peg3, the protein composition of the g-exonemes remains unclear. Here, we used high-resolution imaging and BioID methods to study the two types of egress vesicles in Plasmodium falciparum gametocytes. We show that OB exocytosis precedes discharge of the g-exonemes and that exocytosis of the g-exonemes, but not of the OBs, is calcium-sensitive. Further, the two types of vesicles exhibit distinct proteomes. In addition to known egress-related proteins, our analyses revealed novel markers of OBs and g-exonemes, including proteins involved in vesicle trafficking. Our data provide insight into the immense molecular machinery required for the inside-out egress of P. falciparum gametocytes.
HostingRepository	jPOST
AnnounceDate	2024-01-25
AnnouncementXML	Submission_2024-01-24_07:00:19.223.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Malte Sielaff
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-26 00:33:58	ID requested
⏵ 1	2024-01-24 07:00:19	announced
2	2024-01-24 07:10:27	announced	2024-01-25: Updated PubMed.

Dataset with its publication pending

submitter keyword: Plasmodium falciparum, malaria, gametocyte, egress, red blood cell, vesicle

Stefan Tenzer
lab head
Malte Sielaff
contact affiliation	University Medical Center of the Johannes Gutenberg-University Mainz
dataset submitter

jPOST dataset URI

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