PXD039671-1

PXD039671 is an original dataset announced via ProteomeXchange.

Title	Regulation of host RNA splicing in Mycobacterium tuberculosis infected macrophages
Description	Alternative splicing is a key event which enables us to generate protein diversity from a limited set of genetic information. Previously we have shown that Mycobacterium tuberculosis (Mtb) which primarily infects the host macrophages and causes genome-wide transcriptional reprogramming in the host and virulent strain of Mtb H37Rv cause massive alteration in the host RNA splicing that affects the host-cell physiology and helps in its better survival in the host. Here using a high-throughput yeast two-hybrid and mass spectrometry approach we show that mycobacterial proteins can access the RNA splicing machinery of the host, thereby playing a critical role in infection-induced perturbation of alternative splicing. We show that infection with H37Rv or drug-resistant clinical strains results in increased expression of truncated splice variants of a large number of protein-coding genes. These splice variants either do not undergo translation or result in truncated/non-functional proteins upon translation. A more detailed mechanistic understanding of this critical regulatory event in macrophages upon Mtb infection could unravel newer ways to target tuberculosis.
HostingRepository	PRIDE
AnnounceDate	2025-06-17
AnnouncementXML	Submission_2025-06-17_07:22:58.484.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dhiraj Kumar
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 09:11:38	ID requested
⏵ 1	2025-06-17 07:22:58	announced

Dataset with its publication pending

submitter keyword: RNA Splicing, Mycobacterium tuberculosis,: Host-pathogen interaction, Spliceosome

Dhiraj Kumar
contact affiliation	Group Leader, Cellular Immunology International Centre for Genetic Engineering and Biotechnology ICGEB Campus Aruna Asaf Ali Marg 110 067 New Delhi, INDIA
contact email	dhiraj@icgeb.res.in
lab head
Dhiraj Kumar
contact affiliation	ICGEB, New Delhi
contact email	dhiraj@icgeb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD039671

PRIDE project URI

• PRIDE
  1. PXD039671
     1. Label: PRIDE project
     2. Name: Regulation of host RNA splicing in Mycobacterium tuberculosis infected macrophages