PXD039665 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteome of hand eczema assessed by tape stripping |
Description | Hand eczema (HE) is a prevalent skin disease. However, classification of HE into different subtypes remains challenging. Limited number of prior studies have employed invasive biopsy-based strategies; yet, studies of the HE proteome using non-invasive tape stripping methodology have not been reported. In this study, we wanted to assess whether global proteomic analysis of skin tape strip samples can be used for sub-classification of HE patients. Tape strips were collected from patients with HE and healthy skin. Liquid chromatography–mass spectrometry (LC/MS) proteomics was performed, and the global protein expression was analyzed. We identified 2,919 proteins in stratum corneum-derived skin cells from tape strip samples. Compared to healthy skin, the lesional samples from HE patients exhibited increased expression of immune-related markers and a decreased expression of structural barrier proteins. The difference between HE subtypes was restricted to the lesional skin areas, and included an increased expression of skin barrier-related proteins independently of the concurrent AD. In conclusion we found, that the non-invasive tape strip method used in combination with LC/MS proteomics can be used for analysis of skin protein expression in HE patients. Thus, the method shows potential for assessing the proteomic differences between subtypes of HE, and biomarker discovery. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:45:04.304.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julie Breinholt Kjær Sølberg |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-25 08:25:09 | ID requested | |
1 | 2023-04-26 16:36:50 | announced | |
⏵ 2 | 2023-11-14 08:45:07 | announced | 2023-11-14: Updated project metadata. |
Publication List
S, ø, lberg JBK, Quaade AS, Drici L, Sulek K, Ulrich NH, L, ø, vendorf MB, Thyssen JP, Mann M, Dyring-Andersen B, Johansen JD, The Proteome of Hand Eczema Assessed by Tape Stripping. J Invest Dermatol, 143(8):1559-1568.e5(2023) [pubmed] |
Keyword List
submitter keyword: proteome, subtypes, atopic dermatitis,Hand eczema, Tape stripping |
Contact List
Beatrice Dyring-Andersen |
contact affiliation | Novo Nordisk Foundation (NNF) Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark, and Department of Dermatology and Allergy Herlev-Gentofte Hospital, University of Copenhagen Hellerup, Denmark. |
contact email | beatrice.dyring-andersen@regionh.dk |
lab head | |
Julie Breinholt Kjær Sølberg |
contact affiliation | Department of allergy and skin, Gentofte Hospital, Gentofte |
contact email | julie.soelberg@regionh.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039665
- Label: PRIDE project
- Name: The proteome of hand eczema assessed by tape stripping