PXD039664-1

PXD039664 is an original dataset announced via ProteomeXchange.

Title	Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes
Description	Migrasomes are newly discovered cell organelles forming by local swelling of retraction fibers. The migrasome formation critically depends on tetraspanin proteins present in the retraction fiber membranes and is modulated by the membrane tension and bending rigidity. It remained unknown how and in which time sequence these factors are involved in migrasome nucleation, growth, and stabilization, and what are the possible intermediate stages of migrasome biogenesis. Here using live cell imaging and biomimetic system for migrasomes and retraction fibers, we reveal that migrasome formation is a two-stage process. At the first stage, which in biomimetic system is mediated by membrane tension, local swellings largely devoid of tetraspanin 4 form on the retraction fibers. At the second stage, tetraspanin 4 molecules migrate toward and onto these swellings, which grow up to several microns in size and transform into migrasomes. This tetraspanin 4 recruitment to the swellings is essential for migrasome growth and stabilization. Based on these findings we propose that the major role of tetraspanin proteins is in stabilizing the migrasome structure while the migrasome nucleation and initial growth stages can be driven by membrane mechanical stresses.
HostingRepository	PRIDE
AnnounceDate	2023-01-29
AnnouncementXML	Submission_2023-01-28_23:56:01.707.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TamarZiv
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 08:16:57	ID requested
⏵ 1	2023-01-28 23:56:02	announced

Dataset with its publication pending

submitter keyword: Migrasome,Tetraspanin 4

RayaSorkin
contact affiliation	School of Chemistry, Raymond & Beverly Sackler Faculty of Exact Sciences and Center for Physics and Chemistry of Living Systems, Tel Aviv University, Tel Aviv, Israel
contact email	rsorkin@tauex.tau.ac.il
lab head
TamarZiv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039664
     1. Label: PRIDE project
     2. Name: Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes