PXD039657-3

PXD039657 is an original dataset announced via ProteomeXchange.

Title	PALEOPROTEOMICS APPLIED TO THE WAZIERS SITE
Description	The ancient preserved molecules in bones offer the opportunity to gain a better knowledge on the biological past. In recent years, bones proteomics has become an attractive method to study extinct species and phylogenetic evolution as an alternative to DNA analysis which is limited by DNA amplification present in ancient samples and its contamination. The analysis of fossils must consume a low quantity of material to avoid damaging the samples. Another difficulty is the absence of genomic data for most of the extinct species. This study applied a proteomic methodology to mammalian bones of 130,000 / 120,000 years old from the last Chibanian and the earlier Upper Pleistocene site of Waziers (France). The presence of lithic artefacts and the traces of butchery observed on the bones indicate the presence of Neanderthal Man on this site. Starting from 5 milligram samples, our results show that most detected peptides match collagen I alpha 1 and alpha 2 proteins with a sequence coverage up to 60 %. Using sequence homology with modern sequences, a biological classification was successfully achieved. Each bone taxonomic rank by proteomics was consistent with the existing osteomorphological studies and palaeoenvironmental and palaeodietary data.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:47:26.507.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BRAY FABRICE
SpeciesList	scientific name: Castor; NCBI TaxID: 10184; scientific name: Rhinoceros; NCBI TaxID: 9808; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Cygnus; NCBI TaxID: 8867; scientific name: Elephantidae; NCBI TaxID: 9780; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; scientific name: Cervus sp.; NCBI TaxID: 27598; scientific name: Lutra; NCBI TaxID: 9656;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 06:22:54	ID requested
1	2023-04-20 00:26:38	announced
2	2023-04-20 03:19:35	announced	2023-04-20: Updated project metadata.
⏵ 3	2023-11-14 06:47:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: mammalian bones, taxonomy,Palaeoproteomics, Pleistocene, post translational modifications., Waziers

fabrice bray
contact affiliation	Laboratoire MSAP UAR 3290 CNRS Bâtiment C4 Avenue Paul Langevin Cité Scientifique 59655 Villeneuve D'ascq
contact email	fabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliation	MSAP USR 3290
contact email	fabrice.bray@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD039657

PRIDE project URI

• PRIDE
  1. PXD039657
     1. Label: PRIDE project
     2. Name: PALEOPROTEOMICS APPLIED TO THE WAZIERS SITE