PXD039643-1

PXD039643 is an original dataset announced via ProteomeXchange.

Title	Glycerophospholipid and amino acid metabolism impairment during Congenital Zika Syndrome development
Description	Zika virus (ZIKV) can be transmitted vertically to the fetus, having a high chance to develop Congenital Zika Syndrome (CZS). Studies have shown that ZIKV impairs brain development. However, little is known about the molecular differences between non- microcephalic ZIKV-infected fetuses and microcephalic ones. Here, our main goal is to identify the alterations in the amniotic fluid (AF) metabolome in ZIKV-infected patients and their relation to CZS progression. We applied an untargeted metabolomics strategy to analyze seven AF of pregnant women: healthy women and ZIKV-infected women bearing non-microcephalic and microcephalic fetuses. Infected patients were characterized by glycerophospholipid metabolism impairment, which is accentuated in microcephalic phenotypes. Glycerophospholipid decreased concentration in AF can be a consequence of intracellular transport of lipids to the placental or fetal tissues under development. The increased intracellular concentration of lipids can lead to mitochondrial dysfunction and neurodegeneration caused by lipid droplet accumulation. Furthermore, the dysregulation of amino acid metabolism was a molecular fingerprint of microcephalic phenotypes, specifically serine, and proline metabolisms. Both amino acid deficiencies were related to neurodegenerative disorders, intrauterine growth retardation, and placental abnormalities. This study contributes to the understanding of CZS pathology and discovering potential biomarkers for CZS prognosis in the early stages of pregnancy.
HostingRepository	PRIDE
AnnounceDate	2025-09-04
AnnouncementXML	Submission_2025-09-04_11:14:07.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Unit
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-25 03:31:24	ID requested
⏵ 1	2025-09-04 11:14:07	announced

10.1002/PRCA.202300008;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: metabolomics, Congenital Zika Syndrome, amino acid metabolism,Zika virus, glycerophospholipid metabolism, amniotic fluid

Fábio César Sousa Nogueira
contact affiliation	Laboratory of Protein Chemistry - Proteomic Unit Department of Biochemistry, Institute of Chemistry, UFRJ Av. Athos da Silveira Ramos, 149, CT, Bl. A, 543, Cidade Universitária, CEP 21941-909, Rio de Janeiro, RJ, Brazil
contact email	fabiocsn@gmail.com
lab head
Proteomics Unit
contact affiliation	UFRJ
contact email	unidadeproteomica@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD039643

PRIDE project URI

• PRIDE
  1. PXD039643
     1. Label: PRIDE project
     2. Name: Glycerophospholipid and amino acid metabolism impairment during Congenital Zika Syndrome development