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PXD039530-1

PXD039530 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAutophagy controls the protein composition of hair shafts
DescriptionHair shafts are formed by terminal differentiation of hair keratinocytes (trichocytes) in which keratins and keratin-associated proteins accumulate and undergo cross-linking. Here we tested the hypothesis that maturation of the hair shaft also involves the coordinated degradation of other proteins and, specifically, that this degradation is mediated by autophagy. To this end, we deleted the non-redundant autophagy regulator Atg7 in keratinocytes of the epidermis and skin appendages, including hair, and determined the proteome of hair shafts from fully autophagy-competent and epithelial autophagy-deficient mice. The abrogation of autophagy led to significantly increased abundance of proteins regulating house-keeping functions of the cell and a decrease of cytoskeletal proteins. Translation factors, tRNA-ligases, ribosomal proteins and the components of proteasomes were particularly elevated in the absence of autophagy, indicating a central role of autophagy in regulating multiple steps of protein turnover in hair keratinocytes. These results demonstrate that hair keratinocytes depend on autophagy for establishing the mature protein composition of hair.
HostingRepositoryMassIVE
AnnounceDate2024-07-16
AnnouncementXMLSubmission_2024-07-16_15:30:32.606.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrett Phinney
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListOxidation; Carbamidomethyl; Deamidated
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-17 10:49:12ID requested
12024-07-16 15:30:32announced
Publication List
no publication
Keyword List
submitter keyword: hair, autophagy, cornification, differentiation, keratinocytes
Contact List
Robert H. Rice
contact affiliationDepartment of Environmental Toxicology University of California Davis
contact emailrhrice@ucdavis.edu
lab head
Brett Phinney
contact affiliationUC Davis
contact emailbrettsp@ucdavis.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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