PXD039502 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | piRNA processing by a trimeric Schlafen-domain-containing nuclease |
Description | Transposable elements are genomic parasites that expand within and spread between genomes. Piwi proteins control transposon activity, notably in the germline. These proteins recognize their targets through small RNA co-factors named piRNAs, making piRNA biogenesis a key specificity-determining step in this important genome immunity system. While the processing of piRNA precursors is an essential step in this process, many molecular details of this process remain unknown. We identify a novel endoribonuclease, PUCH, that initiates piRNA processing in the nematode Caenorhabditis elegans. Genetic and biochemical studies show that PUCH, a trimer of Schlafen-like-domain (SLFNL) proteins, executes 5ï‚¢-end piRNA precursor cleavage. PUCH-mediated processing strictly requires an m7G-Cap and a Uracil at position three. We also demonstrate how PUCH interacts with PETISCO, a complex that binds piRNA precursors, and that this interaction enhances piRNA production in vivo. The identification of PUCH completes the C. elegans piRNA biogenesis repertoire and uncovers a novel type of RNA endonuclease formed by three SLFL proteins. Mammalian Slfn genes have been associated with immunity responses, exposing a novel molecular link between immune responses in mammals and deeply conserved RNA-based mechanisms that control transposable elements. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-24 |
AnnouncementXML | Submission_2023-10-24_11:46:10.845.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | FButter |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-16 15:56:48 | ID requested | |
⏵ 1 | 2023-10-24 11:46:11 | announced | |
2 | 2023-11-14 09:07:59 | announced | 2023-11-14: Updated project metadata. |
3 | 2024-10-22 06:09:18 | announced | 2024-10-22: Updated project metadata. |
Publication List
Podvalnaya N, Bronkhorst AW, Lichtenberger R, Hellmann S, Nischwitz E, Falk T, Karaulanov E, Butter F, Falk S, Ketting RF, piRNA processing by a trimeric Schlafen-domain nuclease. Nature, 622(7982):402-409(2023) [pubmed] |
Keyword List
submitter keyword: interactomics |
LC-MS/MS |
Contact List
FalkButter |
contact affiliation | IMB, Ackermannweg 4, 55128 Mainz |
contact email | f.butter@imb.de |
lab head | |
FButter |
contact affiliation | Quantitative Proteomics Institute of Molecular Biology (IMB) |
contact email | f.butter@imb-mainz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039502
- Label: PRIDE project
- Name: piRNA processing by a trimeric Schlafen-domain-containing nuclease