PXD039423 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Preimplantation mouse embryo proteome during 8C to 16C division |
Description | Preimplantation stages of mouse embryo development involve temporal and spatial specification and segregation of three late blastocyst cell lineages; trophectoderm (TE), primitive endoderm (PrE) and epiblast (EPI). Spatial separation of the outer TE lineage from the two inner-cell-mass (ICM) lineages (PrE and EPI) starts with the 8- to 16-cell transition and concludes following transit through the 16- to 32-cell stages. This results in a nascent early blastocyst ICM derived from descendants of primary founding inner cells and a secondarily contributed population; of which subsequent relative EPI versus PrE potencies are subject to debate. We showed that generation of primary but not the secondary ICM populations is highly dependent on temporally discreet activation of the mammalian target of rapamycin (mTOR – specifically mTORC1) around 8-cell stage M-phase entry. Moreover, that this role is mediated via the regulated function of the 7-methylguanosine- (7mG) cap binding initiation complex (EIF4F) and potentiating the translation of a subset of key but otherwise intransigent mRNAs containing 5’ UTR terminal oligopyrimidine (TOP-) sequence motifs. To find out translation of which mRNAs is regulated by mTOR during 8- to 16-cell transition, we analysed proteomes of control and mTOR-inhibited embryos during this time window. |
HostingRepository | PRIDE |
AnnounceDate | 2023-08-18 |
AnnouncementXML | Submission_2023-08-18_05:11:40.534.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | DavidPotesil |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-13 07:24:18 | ID requested | |
⏵ 1 | 2023-08-18 05:11:40 | announced | |
2 | 2023-11-14 07:01:27 | announced | 2023-11-14: Updated project metadata. |
3 | 2024-10-22 06:00:36 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: mouse preimplantation embryos, cap-dependent translation, mTOR |
Contact List
ZbynekZdrahal |
contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
contact email | zdrahal@sci.muni.cz |
lab head | |
DavidPotesil |
contact affiliation | Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic |
contact email | david.potesil@ceitec.muni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039423
- Label: PRIDE project
- Name: Preimplantation mouse embryo proteome during 8C to 16C division