PXD039409-2

PXD039409 is an original dataset announced via ProteomeXchange.

Title	Characterization of spatial heterogeneity in metastasized colorectal cancer by MALDI imaging
Description	About 50% of colorectal cancer patients develop liver metastases. Patients with metastatic colorectal cancer have 5-year survival rates below 20% despite new therapeutic regimens. Tumor heterogeneity has been linked with poor clinical outcome, but was so far mainly studied via bulk genomic analyses. In this study we performed spatial proteomics via MALDI mass spectrometry imaging on six patient matched CRC primary tumor and liver metastases to characterize interpatient, intertumor and intratumor hetereogeneity. We found several peptide features that were enriched in vital tumor areas of primary tumors and liver metastasis and tentatively derived from tumor cell specific proteins such as annexin A4 and prelamin A/C. Liver metastases of colorectal cancer showed higher heterogeneity between patients than primary tumors while within patients both entities show similar intratumor heterogeneity sometimes organized in zonal pattern. Together our findings give new insights into the spatial proteomic heterogeneity of primary CRC and patient matched liver metastases.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:42:09.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lennart Moritz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Plus MALDI TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-13 06:45:27	ID requested
1	2023-02-17 14:15:23	announced
⏵ 2	2024-10-22 05:42:09	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: colorectal cancer

metastasis

tumor heterogeneity

spatial proteomics

mass spectrometry imaging

MALDI imaging

formalin-fixed paraffin-embedded tissues

Melanie Föll
contact affiliation	Institute for Surgical Pathology, Medical Center and Faculty of Medicine - University of Freiburg, Freiburg, Germany
contact email	melanie.foell@mol-med.uni-freiburg.de
lab head
Lennart Moritz
contact affiliation	Universitätsklinik Freiburg
contact email	lennart.moritz@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039409

PRIDE project URI

• PRIDE
  1. PXD039409
     1. Label: PRIDE project
     2. Name: Characterization of spatial heterogeneity in metastasized colorectal cancer by MALDI imaging